GSP1/YLR293C Summary Help

Standard Name GSP1 1
Systematic Name YLR293C
Alias CNR1 2 , CST17 3
Feature Type ORF, Verified
Description Ran GTPase; GTP binding protein (mammalian Ranp homolog) involved in the maintenance of nuclear organization, RNA processing and transport; regulated by Srm1p, Rna1p, Yrb1p, Yrb2p, Yrp4p, Yrb30p, Cse1p and Kap95p; GSP1 has a paralog, GSP2, that arose from the whole genome duplication (1, 2, 4, 5, 6, 7, 8, 9, 10)
Name Description Genetic Suppressor of Prp20-1 1
Chromosomal Location
ChrXII:721430 to 720771 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All GSP1 GO evidence and references
  View Computational GO annotations for GSP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 11 genes
Classical genetics
Large-scale survey
206 total interaction(s) for 78 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 39
  • Affinity Capture-RNA: 7
  • Affinity Capture-Western: 41
  • Biochemical Activity: 5
  • Co-fractionation: 1
  • Co-purification: 3
  • Far Western: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 61
  • Two-hybrid: 30

Genetic Interactions
  • Dosage Rescue: 14
  • Synthetic Growth Defect: 2
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 219
Molecular Weight (Da) 24,810
Isoelectric Point (pI) 6.52
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:721430 to 720771 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..660 721430..720771 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004284
References cited on this page View Complete Literature Guide for GSP1
1) Belhumeur P, et al.  (1993) GSP1 and GSP2, genetic suppressors of the prp20-1 mutant in Saccharomyces cerevisiae: GTP-binding proteins involved in the maintenance of nuclear organization. Mol Cell Biol 13(4):2152-61
2) Kadowaki T, et al.  (1993) Regulation of RNA processing and transport by a nuclear guanine nucleotide release protein and members of the Ras superfamily. EMBO J 12(7):2929-37
3) Ouspenski II, et al.  (1995) Ran-binding protein-1 is an essential component of the Ran/RCC1 molecular switch system in budding yeast. J Biol Chem 270(5):1975-8
4) Schlenstedt G, et al.  (1997) Yrb4p, a yeast ran-GTP-binding protein involved in import of ribosomal protein L25 into the nucleus. EMBO J 16(20):6237-49
5) Kunzler M and Hurt EC  (1998) Cse1p functions as the nuclear export receptor for importin alpha in yeast. FEBS Lett 433(3):185-90
6) Becker J, et al.  (1995) RNA1 encodes a GTPase-activating protein specific for Gsp1p, the Ran/TC4 homologue of Saccharomyces cerevisiae. J Biol Chem 270(20):11860-5
7) Lee A, et al.  (1994) Site-directed mutagenesis of the yeast PRP20/SRM1 gene reveals distinct activity domains in the protein product. Mol Gen Genet 245(1):32-44
8) Schlenstedt G, et al.  (1995) Mutants in a yeast Ran binding protein are defective in nuclear transport. EMBO J 14(21):5367-78
9) Floer M and Blobel G  (1996) The nuclear transport factor karyopherin beta binds stoichiometrically to Ran-GTP and inhibits the Ran GTPase activating protein. J Biol Chem 271(10):5313-6
10) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61