SEC22/YLR268W Summary Help

Standard Name SEC22 1
Systematic Name YLR268W
Alias SLY2 , TSL26 , TS26 2
Feature Type ORF, Verified
Description R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog (3, 4)
Name Description SECretory 5
Chromosomal Location
ChrXII:680200 to 680844 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SEC22 GO evidence and references
  View Computational GO annotations for SEC22
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
conditional
null
Large-scale survey
conditional
null
Resources
895 total interaction(s) for 463 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 14
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 43
  • Co-fractionation: 5
  • Co-purification: 3
  • FRET: 3
  • PCA: 5
  • Reconstituted Complex: 25
  • Two-hybrid: 16

Genetic Interactions
  • Dosage Rescue: 25
  • Negative Genetic: 498
  • Phenotypic Enhancement: 48
  • Phenotypic Suppression: 70
  • Positive Genetic: 73
  • Synthetic Growth Defect: 31
  • Synthetic Lethality: 29
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 214
Molecular Weight (Da) 25,058
Isoelectric Point (pI) 7.34
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:680200 to 680844 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..645 680200..680844 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004258
References cited on this page View Complete Literature Guide for SEC22
1) Novick P, et al.  (1980) Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell 21(1):205-15
2) Hasegawa H, et al.  (1989) Isolation, DNA sequence and regulation of a new cell division cycle gene from the yeast Saccharomyces cerevisiae. Yeast 5(6):509-24
3) Liu Y and Barlowe C  (2002) Analysis of Sec22p in endoplasmic reticulum/Golgi transport reveals cellular redundancy in SNARE protein function. Mol Biol Cell 13(9):3314-24
4) Liu Y, et al.  (2004) Sec22p export from the endoplasmic reticulum is independent of SNARE pairing. J Biol Chem 279(26):27225-32
5) Novick P and Schekman R  (1979) Secretion and cell-surface growth are blocked in a temperature-sensitive mutant of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 76(4):1858-62