Standard Name	ECM22 1
Systematic Name	YLR228C
Feature Type	ORF, Verified
Description	Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication (2, 3, 4, 5)
Name Description	ExtraCellular Mutant

Chromosomal Location	ChrXII:602463 to 600019 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for ECM22
Molecular Function
Manually curated	RNA polymerase II core promoter proximal region sequence-specific DNA binding (IDA) RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription (IDA, IMP)
High-throughput	sequence-specific DNA binding (IDA)
Biological Process
Manually curated	positive regulation of ergosterol biosynthetic process by positive regulation of transcription from RNA polymerase II promoter (IMP) positive regulation of sterol import by positive regulation of transcription from RNA polymerase II promoter (IGI)
Cellular Component
Manually curated	membrane (IDA) nucleus (IC) perinuclear region of cytoplasm (IDA)

Binding motifs	CGGGNNNNCGGA 6 [Search intergenic regions for CGGGNNNNCGGA] HTCCGGA 7 [Search intergenic regions for HTCCGGA]
Regulatory modules	predicted: stressResponse (373)
Resources	ScerTF | UniProbe | YEASTRACT | YeTFaSCo

Classical genetics
null	ergosterol accumulation: decreased resistance to caffeine: decreased resistance to fluconazole: decreased viable
overexpression	budding index: abnormal cell cycle progression in G1 phase: abnormal cell size: increased cellular morphology: abnormal
Large-scale survey
null	competitive fitness: increased haploinsufficient resistance to 4-nitroquinoline N-oxide: normal resistance to cycloheximide: decreased resistance to oligomycin: normal resistance to rhodamine 6-G: normal resistance to benzo[a]pyrene: decreased resistance to ketoconazole: normal resistance to chloramphenicol: normal viable
overexpression	vegetative growth: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	56 total interaction(s) for 48 unique genes/features.
Physical Interactions	Affinity Capture-MS: 4 Affinity Capture-RNA: 2 Affinity Capture-Western: 1 Biochemical Activity: 2
Genetic Interactions	Dosage Growth Defect: 2 Dosage Lethality: 2 Negative Genetic: 26 Positive Genetic: 2 Synthetic Growth Defect: 4 Synthetic Lethality: 9 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXII:602463 to 600019 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2445 602463..600019 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000004218