PBA1/YLR199C Summary Help

Standard Name PBA1 1
Systematic Name YLR199C
Alias POC1 2
Feature Type ORF, Verified
Description Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; interaction with Pba1p-Add66p may affect function of the mature proteasome and its role in maintaining respiratory metabolism; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly (1, 3)
Name Description Proteasome Biogenesis-Associated 1
Chromosomal Location
ChrXII:548769 to 547853 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PBA1 GO evidence and references
  View Computational GO annotations for PBA1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 6 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
44 total interaction(s) for 32 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 18
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 3
  • Co-crystal Structure: 1
  • Co-fractionation: 2
  • Reconstituted Complex: 1

Genetic Interactions
  • Negative Genetic: 11
  • Positive Genetic: 1
  • Synthetic Growth Defect: 4
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 276
Molecular Weight (Da) 30,695
Isoelectric Point (pI) 4.53
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:548769 to 547853 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2003-09-27
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..6 548769..548764 2011-02-03 2003-09-27
Intron 7..92 548763..548678 2011-02-03 2003-09-27
CDS 93..917 548677..547853 2011-02-03 2003-09-27
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004189
References cited on this page View Complete Literature Guide for PBA1
1) Li X, et al.  (2007) beta-Subunit appendages promote 20S proteasome assembly by overcoming an Ump1-dependent checkpoint. EMBO J 26(9):2339-49
2) Le Tallec B, et al.  (2007) 20S proteasome assembly is orchestrated by two distinct pairs of chaperones in yeast and in mammals. Mol Cell 27(4):660-74
3) Stadtmueller BM, et al.  (2012) Structure of a proteasome Pba1-Pba2 complex: implications for proteasome assembly, activation, and biological function. J Biol Chem 287(44):37371-82