ASP3-3/YLR158C Summary

Standard Name	ASP3-3 (see Repeated Note)
Systematic Name	YLR158C
Alias	ASP3
Feature Type	ORF, Verified
Description	Cell-wall L-asparaginase II involved in asparagine catabolism; expression induced during nitrogen starvation; ORF contains a short non-coding RNA that enhances expression of full-length gene; likely arose in via horizontal gene transfer from the wine yeast Wickerhamomyces anomalus or a close relative; reference strain S288C has four copies of ASP3; ASP3-3 has a paralog, ASP3-1, that arose from a segmental duplication (1, 2, 3, 4, 5 and see Summary Paragraph)
Name Description	ASParaginase

Chromosomal Location	ChrXII:483637 to 482549 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for ASP3-3
Molecular Function
Manually curated	asparaginase activity (IDA)
Biological Process
Manually curated	asparagine catabolic process (IDA) cellular response to nitrogen starvation (IDA)
Cellular Component
Manually curated	cell wall-bounded periplasmic space (IDA)

Pathways	asparagine degradation

Large-scale survey
overexpression	resistance to sirolimus: increased vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

	23 total interaction(s) for 23 unique genes/features.
Genetic Interactions	Negative Genetic: 20 Positive Genetic: 3
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DRYGIN \| GeneMANIA \| InterologFinder \| InterologFinder \| InterologFinder \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Localization	YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXII:483637 to 482549 | |

Note: this feature is encoded on the Crick strand.

This feature is contained within: 70945

This feature is contained within: 70081

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1089	483637..482549	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP at NCBI \| BLASTP at NCBI \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000004148

REPEATED NOTE

Name	Relevance	Description
ASP3-4	Repeated	There are 4 copies of ASP3 in the genome.
ASP3-2	Repeated	There are 4 copies of ASP3 in the genome.
ASP3-1	Repeated	There are 4 copies of ASP3 in the genome.

SUMMARY PARAGRAPH for ASP3-3

ASP3 encodes a nitrogen catabolite-regulated cell wall L-asparaginase II (1). It is one of two asparaginases in yeast, the other being the constitutive intracellular asparaginase I Asp1p, with which Asp3p shares 46% identity (6). Asp3p catalyzes the conversion of L-asparagine to aspartate and ammonia. Asp3p is secreted in response to nitrogen starvation and appears to be regulated by Gln3p/Ure2p (7). ASP3 is located at the junction on chromosome XII with the rRNA genes and is repeated at least four times, to create ASP3-1, ASP3-2, ASP3-3, ASP3-4.

Last updated: 1999-09-01

1)	Kim KW, et al. (1988) Asparaginase II of Saccharomyces cerevisiae. Characterization of the ASP3 gene. J Biol Chem 263(24):11948-53
2)	Oliveira EM, et al. (2003) The role of the GATA factors Gln3p, Nil1p, Dal80p and the Ure2p on ASP3 regulation in Saccharomyces cerevisiae. Yeast 20(1):31-7
3)	Katju V, et al. (2009) Variation in gene duplicates with low synonymous divergence in Saccharomyces cerevisiae relative to Caenorhabditis elegans. Genome Biol 10(7):R75
4)	Huang YC, et al. (2010) Intragenic transcription of a noncoding RNA modulates expression of ASP3 in budding yeast. RNA 16(11):2085-93
5)	League GP, et al. (2012) The ASP3 locus in Saccharomyces cerevisiae originated by horizontal gene transfer from Wickerhamomyces. FEMS Yeast Res 12(7):859-63
6)	Sinclair K, et al. (1994) The ASP1 gene of Saccharomyces cerevisiae, encoding the intracellular isozyme of L-asparaginase. Gene 144(1):37-43
7)	Bon EP, et al. (1997) Asparaginase II of Saccharomyces cerevisiae. GLN3/URE2 regulation of a periplasmic enzyme. Appl Biochem Biotechnol 63-65():203-12