ACE2/YLR131C Summary Help

Standard Name ACE2 1
Systematic Name YLR131C
Feature Type ORF, Verified
Description Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
Name Description Activator of CUP1 Expression 1
Chromosomal Location
ChrXII:406822 to 404510 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All ACE2 GO evidence and references
  View Computational GO annotations for ACE2
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulatory Role
Binding motifs Predicted ACE2 Binding Site Locations
Resources
Mutant phenotypes All ACE2 Phenotype evidence and references
Classical genetics
null
Large-scale survey
null
overexpression
Resources
interactions All ACE2 Interaction evidence and references
148 total interaction(s) for 121 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-Western: 3
  • Biochemical Activity: 5
  • Protein-peptide: 1
  • Protein-RNA: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 14
Genetic Interactions
  • Dosage Rescue: 6
  • Negative Genetic: 78
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 7
  • Positive Genetic: 6
  • Synthetic Growth Defect: 3
  • Synthetic Rescue: 3
Resources
Expression Summary
histogram
Resources
Protein Information All ACE2 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:406822 to 404510 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..2313 406822..404510 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004121
References cited on this page View Complete Literature Guide for ACE2
1) Butler G and Thiele DJ  (1991) ACE2, an activator of yeast metallothionein expression which is homologous to SWI5. Mol Cell Biol 11(1):476-85
2) O'Conallain C, et al.  (1999) Regulated nuclear localisation of the yeast transcription factor Ace2p controls expression of chitinase (CTS1) in Saccharomyces cerevisiae. Mol Gen Genet 262(2):275-82
3) Dohrmann PR, et al.  (1996) Role of negative regulation in promoter specificity of the homologous transcriptional activators Ace2p and Swi5p. Mol Cell Biol 16(4):1746-58
4) Dohrmann PR, et al.  (1992) Parallel pathways of gene regulation: homologous regulators SWI5 and ACE2 differentially control transcription of HO and chitinase. Genes Dev 6(1):93-104
5) Bidlingmaier S, et al.  (2001) The Cbk1p pathway is important for polarized cell growth and cell separation in Saccharomyces cerevisiae. Mol Cell Biol 21(7):2449-62
6) Laabs TL, et al.  (2003) ACE2 is required for daughter cell-specific G1 delay in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 100(18):10275-80
7) Nelson B, et al.  (2003) RAM: a conserved signaling network that regulates Ace2p transcriptional activity and polarized morphogenesis. Mol Biol Cell 14(9):3782-803
8) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
9) Sbia M, et al.  (2008) Regulation of the yeast Ace2 transcription factor during the cell cycle. J Biol Chem 283(17):11135-45
10) Mazanka E, et al.  (2008) The NDR/LATS family kinase Cbk1 directly controls transcriptional asymmetry. PLoS Biol 6(8):e203
11) Mazanka E and Weiss EL  (2010) Sequential Counteracting Kinases Restrict an Asymmetric Gene Expression Program to early G1. Mol Biol Cell 21(16):2809-20
12) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87
13) Zhu J and Zhang MQ  (1999) SCPD: a promoter database of the yeast Saccharomyces cerevisiae. Bioinformatics 15(7-8):607-11
14) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104