SDO1/YLR022C Summary

Standard Name	SDO1 1
Systematic Name	YLR022C
Feature Type	ORF, Verified
Description	Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes (1, 2, 3, 4, 5 and see Summary Paragraph)

Chromosomal Location	ChrXII:184926 to 184174 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All SDO1 GO evidence and references

	View Computational GO annotations for SDO1
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	mature ribosome assembly (IMP)
Cellular Component
Manually curated	colocalizes_with polysome (IDA) colocalizes_with preribosome, large subunit precursor (IDA)
High-throughput	cytoplasm (IDA) nucleus (IDA)

Mutant phenotypes All SDO1 Phenotype evidence and references

Classical genetics
null	dessication resistance: increased vegetative growth: decreased rate
Large-scale survey
null	inviable starvation resistance: decreased vegetative growth: decreased
reduction of function	competitive fitness: decreased
unspecified	vegetative growth: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All SDO1 Interaction evidence and references

	50 total interaction(s) for 45 unique genes/features.
Physical Interactions	Affinity Capture-MS: 12 Affinity Capture-RNA: 3 Affinity Capture-Western: 2 Biochemical Activity: 1 PCA: 1 Protein-peptide: 1 Reconstituted Complex: 2 Two-hybrid: 8
Genetic Interactions	Negative Genetic: 12 Synthetic Growth Defect: 2 Synthetic Lethality: 4 Synthetic Rescue: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Mass Spec \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All SDO1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXII:184926 to 184174 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..753	184926..184174	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000004012

SUMMARY PARAGRAPH for SDO1

SDO1 encodes a 60S ribosome maturation factor associated with pre-60S ribosomes and polysomes where it functions in the release and recycling of pre-60S constituent Tif6p. Tif6p is required for efficient processing of rRNA and for pre-60S ribosome nuclear export. Tif6p shuttles between the nucleus and cytoplasm and its release from pre-60S ribosomes is a key-step in 60S maturation and subsequent translational activation of ribosomes (1 and references therein).

Sdo1p is highly conserved among archae and eukaryotes (4). Mutations in the human ortholog, SBDS, cause Shwachman-Diamond syndrome (aka Shwachman-Bodian-Diamond syndrome), an autosomal recessive disease characterized by pancreatic insufficiency, skeletal abnormalities, bone marrow failure and a predisposition to leukemia. Another S. cerevisiae protein, YHR087W, contains the same unusual structural domain found in SBDS and orthologs, but does not display similarity beyond this domain (2 and 3).

The sdo1 deletion mutant has been variously reported both as inviable (6) and as having a slow-growth phenotype (1).

Last updated: 2007-03-26

References cited on this page View Complete Literature Guide for SDO1

1)	Menne TF, et al. (2007) The Shwachman-Bodian-Diamond syndrome protein mediates translational activation of ribosomes in yeast. Nat Genet 39(4):486-95
2)	Shammas C, et al. (2005) Structural and mutational analysis of the SBDS protein family. Insight into the leukemia-associated Shwachman-Diamond Syndrome. J Biol Chem 280(19):19221-9
3)	Savchenko A, et al. (2005) The Shwachman-Bodian-Diamond syndrome protein family is involved in RNA metabolism. J Biol Chem 280(19):19213-20
4)	Boocock GR, et al. (2006) Phylogeny, sequence conservation, and functional complementation of the SBDS protein family. Genomics 87(6):758-71
5)	Finch AJ, et al. (2011) Uncoupling of GTP hydrolysis from eIF6 release on the ribosome causes Shwachman-Diamond syndrome. Genes Dev 25(9):917-29
6)	Winzeler EA, et al. (1999) Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 285(5429):901-6