BAS1/YKR099W Summary Help

Standard Name BAS1 1
Systematic Name YKR099W
Feature Type ORF, Verified
Description Myb-related transcription factor; involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes (2, 3, 4 and see Summary Paragraph)
Name Description BASal 1
Chromosomal Location
ChrXI:635851 to 638286 | ORF Map | GBrowse
Genetic position: 82 cM
Gene Ontology Annotations All BAS1 GO evidence and references
  View Computational GO annotations for BAS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 133 genes
Classical genetics
Large-scale survey
138 total interaction(s) for 119 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-Western: 2
  • Protein-RNA: 1
  • Two-hybrid: 11

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 74
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 1
  • Positive Genetic: 39
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 2
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 811
Molecular Weight (Da) 89,603
Isoelectric Point (pI) 8.39
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:635851 to 638286 | ORF Map | GBrowse
Genetic position: 82 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2436 635851..638286 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001807

BAS1 encodes a transcription factor involved in the expression of genes encoding enzymes acting in the histidine, purine, and pyrimidine biosynthetic pathways (5, 6, 1, 7, 1, 3). A genome-wide survey of genes regulated by Bas1p was performed by Denis et al. (5). Bas1p acts in conjunction with Grf10p (1) in a TATA-independent fashion (8). Bas1p has been shown to bind to a site containing a TGACTC hexanucleotide found in the ADE2 and ADE5,7 promoters (9). This motif is required for the regulation of ADE2 expression by Bas1p/Bas2p (3). A model for the action of Bas1p and Bas2p is that formation of a complex between the two proteins unmasks an activator function in Bas1p. Moreover, in adenine-replete cells, there may be a negative regulator that inhibits formation of this complex (10, 11). However, there is evidence that there may be other partners that can bind Bas1p (6). The N-terminal region of Bas1p is similar to those of proteins in the myb proto-oncogene family (2).

Last updated: 1999-07-01 Contact SGD

References cited on this page View Complete Literature Guide for BAS1
1) Arndt KT, et al.  (1987) Multiple global regulators control HIS4 transcription in yeast. Science 237(4817):874-80
2) Tice-Baldwin K, et al.  (1989) BAS1 has a Myb motif and activates HIS4 transcription only in combination with BAS2. Science 246(4932):931-5
3) Daignan-Fornier B and Fink GR  (1992) Coregulation of purine and histidine biosynthesis by the transcriptional activators BAS1 and BAS2. Proc Natl Acad Sci U S A 89(15):6746-50
4) Mieczkowski PA, et al.  (2006) Global analysis of the relationship between the binding of the Bas1p transcription factor and meiosis-specific double-strand DNA breaks in Saccharomyces cerevisiae. Mol Cell Biol 26(3):1014-27
5) Denis V, et al.  (1998) Role of the myb-like protein bas1p in Saccharomyces cerevisiae: a proteome analysis. Mol Microbiol 30(3):557-66
6) Denis V and Daignan-Fornier B  (1998) Synthesis of glutamine, glycine and 10-formyl tetrahydrofolate is coregulated with purine biosynthesis in Saccharomyces cerevisiae. Mol Gen Genet 259(3):246-55
7) Springer C, et al.  (1996) Amino acid and adenine cross-pathway regulation act through the same 5'-TGACTC-3' motif in the yeast HIS7 promoter. J Biol Chem 271(47):29637-43
8) Pellman D, et al.  (1990) TATA-dependent and TATA-independent transcription at the HIS4 gene of yeast. Nature 348(6296):82-5
9) Hovring I, et al.  (1994) DNA-binding domain and recognition sequence of the yeast BAS1 protein, a divergent member of the Myb family of transcription factors. J Biol Chem 269(26):17663-9
10) Zhang F, et al.  (1997) Evidence that complex formation by Bas1p and Bas2p (Pho2p) unmasks the activation function of Bas1p in an adenine-repressible step of ADE gene transcription. Mol Cell Biol 17(6):3272-83
11) Rolfes RJ, et al.  (1997) The transcriptional activators BAS1, BAS2, and ABF1 bind positive regulatory sites as the critical elements for adenine regulation of ADE5,7. J Biol Chem 272(20):13343-54
12) Zhu C, et al.  (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66
13) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104