Standard Name	TRM2 (see Nomenclature conflict Note)
Systematic Name	YKR056W
Alias	NUC2 , NUD1 , RNC1
Feature Type	ORF, Verified
Description	tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair (1, 2, 3)
Name Description	Transfer RNA Methyltransferase

Chromosomal Location	ChrXI:549448 to 551367 | ORF Map | GBrowse

	View Computational GO annotations for TRM2
Molecular Function
Manually curated	double-stranded DNA specific 5'-3' exodeoxyribonuclease activity (IDA) single-stranded DNA specific endodeoxyribonuclease activity (IDA) tRNA (m5U54) methyltransferase activity (IDA, IMP)
Biological Process
Manually curated	DNA repair (IGI, IMP) double-strand break repair (IGI, IMP) tRNA modification (IDA)
Cellular Component
Manually curated	cellular_component unknown (ND)

Classical genetics
null	resistance to methyl methanesulfonate: decreased
Large-scale survey
null	anaerobic growth: decreased competitive fitness: increased resistance to benzo[a]pyrene: decreased viable
overexpression	filamentous growth: decreased invasive growth: absent
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	42 total interaction(s) for 32 unique genes/features.
Physical Interactions	Affinity Capture-MS: 3
Genetic Interactions	Negative Genetic: 26 Phenotypic Enhancement: 4 Positive Genetic: 5 Synthetic Growth Defect: 2 Synthetic Lethality: 1 Synthetic Rescue: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXI:549448 to 551367 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 2004-02-13
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1920 549448..551367 2011-02-03 2004-02-13
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001764