ALY1/YKR021W Summary Help

Standard Name ALY1 1, 2
Systematic Name YKR021W
Alias ART6 3
Feature Type ORF, Verified
Description Alpha arrestin, substrate of calcineurin; controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; dephosphorylation of Aly1p required for the endocytosis of Dip5p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication (3, 4, 5, 6, 7)
Name Description Arrestin-Like Yeast protein 1
Chromosomal Location
ChrXI:479234 to 481981 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All ALY1 GO evidence and references
  View Computational GO annotations for ALY1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
39 total interaction(s) for 25 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 8
  • Biochemical Activity: 6
  • Co-purification: 2
  • Protein-RNA: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 1
  • Phenotypic Enhancement: 1
  • Synthetic Growth Defect: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 915
Molecular Weight (Da) 102,539
Isoelectric Point (pI) 5.93
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:479234 to 481981 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2748 479234..481981 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001729
References cited on this page View Complete Literature Guide for ALY1
1) O' Donnell A  (2006) personal communication
2) Gupta R, et al.  (2007) Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast. Mol Syst Biol 3:116
3) Lin CH, et al.  (2008) Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface. Cell 135(4):714-25
4) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) O'Donnell AF, et al.  (2010) {alpha}-Arrestins Aly1 and Aly2 Regulate Intracellular Trafficking in Response to Nutrient Signaling. Mol Biol Cell 21(20):3552-66
7) O'Donnell AF, et al.  (2013) A Calcineurin-dependent Switch Controls the Trafficking Function of a-Arrestin Aly1/Art6. J Biol Chem 288(33):24063-80