VPS51/YKR020W Summary Help

Standard Name VPS51 1, 2
Systematic Name YKR020W
Alias WHI6 3 , API3 4 , VPS67 5
Feature Type ORF, Verified
Description Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; links the (VFT/GARP) complex to the SNARE Tlg1p; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p, (2, 6, 7, 8)
Name Description Vacuolar Protein Sorting
Chromosomal Location
ChrXI:478338 to 478832 | ORF Map | GBrowse
Gene Ontology Annotations All VPS51 GO evidence and references
  View Computational GO annotations for VPS51
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
reduction of function
Large-scale survey
349 total interaction(s) for 217 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 12
  • Co-crystal Structure: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 181
  • Phenotypic Enhancement: 18
  • Phenotypic Suppression: 64
  • Positive Genetic: 16
  • Synthetic Growth Defect: 21
  • Synthetic Lethality: 20
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 164
Molecular Weight (Da) 18,912
Isoelectric Point (pI) 4.72
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:478338 to 478832 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..495 478338..478832 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001728
References cited on this page View Complete Literature Guide for VPS51
1) Conibear E and Stevens TH  (2000) Vps52p, Vps53p, and Vps54p form a novel multisubunit complex required for protein sorting at the yeast late Golgi. Mol Biol Cell 11(1):305-23
2) Siniossoglou S and Pelham HR  (2002) Vps51p links the VFT complex to the SNARE Tlg1p. J Biol Chem 277(50):48318-24
3) Jorgensen P, et al.  (2002) Systematic identification of pathways that couple cell growth and division in yeast. Science 297(5580):395-400
4) Bidlingmaier S and Snyder M  (2002) Large-scale identification of genes important for apical growth in Saccharomyces cerevisiae by directed allele replacement technology (DART) screening. Funct Integr Genomics 1(6):345-56
5) Bonangelino CJ, et al.  (2002) Genomic screen for vacuolar protein sorting genes in Saccharomyces cerevisiae. Mol Biol Cell 13(7):2486-501
6) Reggiori F, et al.  (2003) Vps51 is part of the yeast Vps fifty-three tethering complex essential for retrograde traffic from the early endosome and Cvt vesicle completion. J Biol Chem 278(7):5009-20
7) Conibear E, et al.  (2003) Vps51p mediates the association of the GARP (Vps52/53/54) complex with the late Golgi t-SNARE Tlg1p. Mol Biol Cell 14(4):1610-23
8) Dotiwala F, et al.  (2013) DNA damage checkpoint triggers autophagy to regulate the initiation of anaphase. Proc Natl Acad Sci U S A 110(1):E41-9