MIA40/YKL195W Summary Help

Standard Name MIA40 1
Systematic Name YKL195W
Alias FMP15 , TIM40 2
Feature Type ORF, Verified
Description Mitochondrial oxidoreductase; involved in mitochondrial intermembrane space import; component of MIA pathway which mediates import and oxidative folding of substrates including small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p via a chaperone-like activity; forms a dimer that binds iron-sulfur cluster in vitro (1, 3, 4, 5, 6)
Name Description Mitochondrial intermembrane space Import and Assembly 1
Chromosomal Location
ChrXI:75821 to 77032 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All MIA40 GO evidence and references
  View Computational GO annotations for MIA40
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 6 genes
Resources
Classical genetics
conditional
null
repressible
Large-scale survey
conditional
null
overexpression
reduction of function
Resources
119 total interaction(s) for 71 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 16
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 22
  • Biochemical Activity: 8
  • Co-fractionation: 1
  • Co-purification: 16
  • Reconstituted Complex: 19

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 2
  • Negative Genetic: 28
  • Phenotypic Enhancement: 1
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 403
Molecular Weight (Da) 44,536
Isoelectric Point (pI) 4.29
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:75821 to 77032 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2003-09-22
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1212 75821..77032 2011-02-03 2003-09-22
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001678
References cited on this page View Complete Literature Guide for MIA40
1) Chacinska A, et al.  (2004) Essential role of Mia40 in import and assembly of mitochondrial intermembrane space proteins. EMBO J 23(19):3735-46
2) Naoe M, et al.  (2004) Identification of Tim40 that mediates protein sorting to the mitochondrial intermembrane space. J Biol Chem 279(46):47815-21
3) Terziyska N, et al.  (2005) Mia40, a novel factor for protein import into the intermembrane space of mitochondria is able to bind metal ions. FEBS Lett 579(1):179-84
4) Mesecke N, et al.  (2005) A disulfide relay system in the intermembrane space of mitochondria that mediates protein import. Cell 121(7):1059-69
5) Weckbecker D, et al.  (2012) Atp23 biogenesis reveals a chaperone-like folding activity of Mia40 in the IMS of mitochondria. EMBO J 31(22):4348-58
6) Spiller MP, et al.  (2013) Identification and characterization of mitochondrial Mia40 as an iron-sulfur protein. Biochem J 455(1):27-35