SDS22/YKL193C Summary Help

Standard Name SDS22 1
Systematic Name YKL193C
Alias EGP1 2
Feature Type ORF, Verified
Description Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function (1, 2, 3, 4, 5, 6, 7 and see Summary Paragraph)
Name Description homolog of S. pombe SDS22 1
Chromosomal Location
ChrXI:79882 to 78866 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SDS22 GO evidence and references
  View Computational GO annotations for SDS22
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulatory Role
Regulatory modules predicted: stressResponse (643, 607, 595, 445, 379)
predicted: cellcycle (341)
predicted: stressResponse (643, 607, 595, 445, 379)
predicted: cellcycle (341)
Mutant phenotypes All SDS22 Phenotype evidence and references
Classical genetics
conditional
Large-scale survey
conditional
null
overexpression
reduction of function
repressible
Resources
interactions All SDS22 Interaction evidence and references
147 total interaction(s) for 105 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 40
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 9
  • Biochemical Activity: 1
  • PCA: 2
  • Reconstituted Complex: 3
  • Two-hybrid: 4
Genetic Interactions
  • Dosage Growth Defect: 5
  • Dosage Lethality: 3
  • Dosage Rescue: 7
  • Negative Genetic: 56
  • Positive Genetic: 5
  • Synthetic Lethality: 7
  • Synthetic Rescue: 4
Resources
Expression Summary
histogram
Resources
Protein Information All SDS22 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:79882 to 78866 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1017 79882..78866 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001676
SUMMARY PARAGRAPH for SDS22

SDS22 encodes a leucine-rich repeat protein that is a positive regulatory subunit of protein phosphatase type 1 (Glc7p) (2). Sds22p forms a stable 1:1 complex with Glc7p (8), and is required for normal nuclear localization of Glc7p (5). Sds22p and Glc7p appear to be involved in regulating microtubule-related functions (2), and seem to function in an interdependent manner (5). Sds22p/Glc7p complexes appear more abundant in early stationary phase extracts than in exponential phase extracts (8), but SDS22 expression appears continuous throughout the cell cycle (2).

SDS22 is an essential gene, and roughly 60% of Sds22p-depleted cells arrest in the large-bud stage of the cell cycle, with approximately 7% of the Sds22p-depleted cells being multinucleate or aploid (2). Some Sds22p-depleted large-budded cells also exhibit abnormal spindle structures (2). Some temperature-sensitive loss-of-function sds22 mutations confer a chromosome loss phenotype at higher growth temperatures (5).

SDS22 is the budding yeast homologue of Schizosaccharomyces pombe sds22 and human SDS22 (5).

Last updated: 2005-09-20

References cited on this page View Complete Literature Guide for SDS22
1) MacKelvie SH, et al.  (1995) The Saccharomyces cerevisiae gene SDS22 encodes a potential regulator of the mitotic function of yeast type 1 protein phosphatase. Mol Cell Biol 15(7):3777-85
2) Hisamoto N, et al.  (1995) The EGP1 gene may be a positive regulator of protein phosphatase type 1 in the growth control of Saccharomyces cerevisiae. Mol Cell Biol 15(7):3767-76
3) Hong G, et al.  (2000) Sds22p is a subunit of a stable isolatable form of protein phosphatase 1 (Glc7p) from Saccharomyces cerevisiae. Arch Biochem Biophys 376(2):288-98
4) Ramaswamy NT, et al.  (1998) Regulation of yeast glycogen metabolism and sporulation by Glc7p protein phosphatase. Genetics 149(1):57-72
5) Peggie MW, et al.  (2002) Essential functions of Sds22p in chromosome stability and nuclear localization of PP1. J Cell Sci 115(Pt 1):195-206
6) Pedelini L, et al.  (2007) YPI1 and SDS22 proteins regulate the nuclear localization and function of yeast type 1 phosphatase Glc7. J Biol Chem 282(5):3282-92
7) Bharucha JP, et al.  (2008) Ypi1, a Positive Regulator of Nuclear Protein Phosphatase Type 1 Activity in Saccharomyces cerevisiae. Mol Biol Cell 19(3):1032-45
8) Walsh EP, et al.  (2002) Novel interactions of Saccharomyces cerevisiae type 1 protein phosphatase identified by single-step affinity purification and mass spectrometry. Biochemistry 41(7):2409-20