DBR1/YKL149C Summary Help

Standard Name DBR1 1
Systematic Name YKL149C
Alias PRP26 2
Feature Type ORF, Verified
Description RNA lariat debranching enzyme; catalyzes debranching of lariat introns formed during pre-mRNA splicing; required for efficient Ty1 transposition; knockdown of human homolog Dbr1 rescues toxicity of RNA-binding proteins TDP-43 and FUS which are implicated in amyotrophic lateral sclerosis (ALS), suggests potential therapeutic target for ALS and related TDP-43 proteinopathies (1, 3)
Name Description DeBRanching 1
Chromosomal Location
ChrXI:168829 to 167612 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -106.4 cM
Gene Ontology Annotations All DBR1 GO evidence and references
  View Computational GO annotations for DBR1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
repressible
Resources
68 total interaction(s) for 58 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Biochemical Activity: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 28
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 6
  • Positive Genetic: 21
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 405
Molecular Weight (Da) 47,741
Isoelectric Point (pI) 7.89
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:168829 to 167612 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: -106.4 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1218 168829..167612 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001632
References cited on this page View Complete Literature Guide for DBR1
1) Chapman KB and Boeke JD  (1991) Isolation and characterization of the gene encoding yeast debranching enzyme. Cell 65(3):483-92
2) Vijayraghavan U, et al.  (1989) Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev 3(8):1206-16
3) Armakola M, et al.  (2012) Inhibition of RNA lariat debranching enzyme suppresses TDP-43 toxicity in ALS disease models. Nat Genet 44(12):1302-9