SLD2/YKL108W Summary 
| Standard Name | SLD2 1 |
|---|---|
| Systematic Name | YKL108W |
| Alias | DRC1 2 |
| Feature Type | ORF, Verified |
| Description | Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress (2, 3, 4, 5, 6, 7, 8, 9, 10) |
| Name Description | Synthetically Lethal with Dpb11-1 1 |
| Chromosomal Location | |
|---|---|
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Gene Ontology Annotations All SLD2 GO evidence and references
| View Computational GO annotations for SLD2 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated | |
| Cellular Component | |
| Manually curated | |
| High-throughput |
Mutant phenotypes All SLD2 Phenotype evidence and references
| Classical genetics | |
|---|---|
| conditional |
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| null | |
| reduction of function | |
| Large-scale survey | |
| null | |
| reduction of function |
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| repressible | |
| Resources |
interactions All SLD2 Interaction evidence and references
| 92 total interaction(s) for 51 unique genes/features. | |
| Physical Interactions |
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| Genetic Interactions |
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| Resources |
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Expression Summary
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| Resources |
Protein Information All SLD2 Protein evidence and references
| Localization | |
|---|---|
| Phosphorylation | PhosphoGRID | PhosphoPep Database |
| Structure | |
| Homologs |
sequence information
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| Last Update | Coordinates: 2011-02-03 | Sequence: 1996-07-31 | ||||||||||||
| Subfeature details |
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| Retrieve sequences | |||||||||||||
Analyze Sequence
| S288C only | |
|---|---|
| S288C vs. other species | |
| S288C vs. other strains |
Resources
| External Links | All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB |
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| Primary SGDID | S000001591 |
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References cited on this page View Complete Literature Guide for SLD2
| 1) | Kamimura Y, et al. (1998) Sld2, which interacts with Dpb11 in Saccharomyces cerevisiae, is required for chromosomal DNA replication. Mol Cell Biol 18(10):6102-9 |
| 2) | Wang H and Elledge SJ (1999) DRC1, DNA replication and checkpoint protein 1, functions with DPB11 to control DNA replication and the S-phase checkpoint in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 96(7):3824-9 |
| 3) | Masumoto H, et al. (2002) S-Cdk-dependent phosphorylation of Sld2 essential for chromosomal DNA replication in budding yeast. Nature 415(6872):651-5 |
| 4) | Ubersax JA, et al. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64 |
| 5) | Tanaka S, et al. (2007) CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast. Nature 445(7125):328-32 |
| 6) | Zegerman P and Diffley JF (2007) Phosphorylation of Sld2 and Sld3 by cyclin-dependent kinases promotes DNA replication in budding yeast. Nature 445(7125):281-5 |
| 7) | Muramatsu S, et al. (2010) CDK-dependent complex formation between replication proteins Dpb11, Sld2, Pol {varepsilon}, and GINS in budding yeast. Genes Dev 24(6):602-12 |
| 8) | Kanter DM and Kaplan DL (2011) Sld2 binds to origin single-stranded DNA and stimulates DNA annealing. Nucleic Acids Res 39(7):2580-92 |
| 9) | Bruck I, et al. (2011) Enabling association of the GINS protein tetramer with the mini chromosome maintenance (Mcm)2-7 protein complex by phosphorylated Sld2 protein and single-stranded origin DNA. J Biol Chem 286(42):36414-26 |
| 10) | Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76 |
