TCD2/YKL027W Summary Help

Standard Name TCD2 1
Systematic Name YKL027W
Feature Type ORF, Verified
Description tRNA threonylcarbamoyladenosine dehydratase; required for the ct6A tRNA base modification, where an adenosine at position 37 is modified to form a cyclized active ester with an oxazolone ring; localized to the mitochondrial outer membrane; TCD2 has a paralog, TCD1, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description tRNA ThreonylCarbamoyladenosine Dehydratase 1
Chromosomal Location
ChrXI:387562 to 388905 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All TCD2 GO evidence and references
  View Computational GO annotations for TCD2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 2 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
overexpression
Resources
55 total interaction(s) for 51 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 3

Genetic Interactions
  • Negative Genetic: 45
  • Phenotypic Suppression: 1
  • Positive Genetic: 2
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 447
Molecular Weight (Da) 50,265
Isoelectric Point (pI) 9.32
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:387562 to 388905 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1344 387562..388905 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001510
References cited on this page View Complete Literature Guide for TCD2
1) Miyauchi K, et al.  (2013) A cyclic form of N6-threonylcarbamoyladenosine as a widely distributed tRNA hypermodification. Nat Chem Biol 9(2):105-11
2) Sickmann A, et al.  (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Zahedi RP, et al.  (2006) Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins. Mol Biol Cell 17(3):1436-50