DID4/YKL002W Summary 
DID4 BASIC INFORMATION
| Standard Name | DID4 1 |
|---|---|
| Systematic Name | YKL002W |
| Alias | GRD7 2 , REN1 3 , VPL2 4 , VPT14 5 , VPS2 5 , 6 , CHM2 7 , VPS14 8 |
| Feature Type | ORF, Verified |
| Description | Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis (1, 3, 9, 10 and see Summary Paragraph) 
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| Name Description | Doa4-Independent Degradation 1 |
| GO Annotations | All DID4 GO evidence and references |
|---|---|
| View Computational GO annotations for DID4 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated | |
| Cellular Component | |
| Manually curated |
| Interactions | DID4 All interactions details and references |
|---|---|
| 38 total interaction(s) for 29 unique genes/features. | |
| Physical Interactions |
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| Genetic Interactions |
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| External Links | All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB |
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| Primary SGDID | S000001485 |
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DID4 RESOURCES
| SGD ORF map | GBrowse |
|---|---|
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Click on histogram for expression summary
Expression Summary
ADDITIONAL INFORMATION for DID4
SUMMARY PARAGRAPH for DID4
Most proteins targeted for degradation in the multivesicular body (MVB) pathway are monoubiquitinated, then passed through the series of highly-conserved multisubunit complexes known as ESCRT (Endosomal Sorting Complex Required for Transport). ESCRT I, II, and III are sequentially mobilized to endosomal membranes, where they direct protein sorting and MVB biogenesis, and play a crucial role in retrovirus budding (11).
The formation of ESCRT III - composed of subcomplexes Snf7p-Vps20p and Did4p-Vps24p - is transient and appears to be dependent on ESCRT II (12, 9). Snf7p-Vps20p effects the membrane association of ESCRT III, with Snf7p being required for localization of the Bro1p vacuolar protein sorting factor to endosomes (10, 13), and Vps20p for formation of ESCRT III (14, 9). The Did4p-Vps24p subcomplex is required for endosomal localization of the Doa4p ubiquitin hydrolase, as well as the Vps4p AAA-type ATPase, which is necessary for the dissociation of ESCRT III (9, 1, 12, 15, 16, 11).
did4 null mutants display growth defects at high temperatures, as well as increased sensitivity to manganese ions. Overproduction of Did4p suppresses the high temperature and canavanine sensitivities of vps24 null mutants (17, 1).
Last updated: 2006-03-13
REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for DID4]
| 1) | Amerik AY, et al. (2000) The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways. Mol Biol Cell 11(10):3365-80 |
| 2) | Nothwehr SF, et al. (1996) The newly identified yeast GRD genes are required for retention of late-Golgi membrane proteins. Mol Cell Biol 16(6):2700-7 |
| 3) | Davis NG, et al. (1993) Cis- and trans-acting functions required for endocytosis of the yeast pheromone receptors. J Cell Biol 122(1):53-65 |
| 4) | Rothman JH and Stevens TH (1986) Protein sorting in yeast: mutants defective in vacuole biogenesis mislocalize vacuolar proteins into the late secretory pathway. Cell 47(6):1041-51 |
| 5) | Robinson JS, et al. (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48 |
| 6) | Rothman JH, et al. (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65 |
| 7) | Howard TL, et al. (2001) CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins. J Cell Sci 114(Pt 13):2395-404 |
| 8) | Raymond CK, et al. (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402 |
| 9) | Babst M, et al. (2002) Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting. Dev Cell 3(2):271-82 |
| 10) | Odorizzi G, et al. (2003) Bro1 is an endosome-associated protein that functions in the MVB pathway in Saccharomyces cerevisiae. J Cell Sci 116(Pt 10):1893-903 |
| 11) | Teo H, et al. (2004) ESCRT-II, an endosome-associated complex required for protein sorting: crystal structure and interactions with ESCRT-III and membranes. Dev Cell 7(4):559-69 |
| 12) | Babst M, et al. (2002) Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body. Dev Cell 3(2):283-9 |
| 13) | Boysen JH and Mitchell AP (2006) Control of Bro1-domain protein Rim20 localization by external pH, ESCRT machinery, and the Saccharomyces cerevisiae Rim101 pathway. Mol Biol Cell 17(3):1344-53 |
| 14) | Ashrafi K, et al. (1998) A role for Saccharomyces cerevisiae fatty acid activation protein 4 in regulating protein N-myristoylation during entry into stationary phase. J Biol Chem 273(40):25864-74 |
| 15) | Yeo SC, et al. (2003) Vps20p and Vta1p interact with Vps4p and function in multivesicular body sorting and endosomal transport in Saccharomyces cerevisiae. J Cell Sci 116(Pt 19):3957-70 |
| 16) | Bowers K, et al. (2004) Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae. Traffic 5(3):194-210 |
| 17) | Eguez L, et al. (2004) Yeast Mn2+ transporter, Smf1p, is regulated by ubiquitin-dependent vacuolar protein sorting. Genetics 167(1):107-17 |
