GRR1/YJR090C Summary Help

Standard Name GRR1 1
Systematic Name YJR090C
Alias CAT80 , COT2 2 , SSU2 3 , SDC1 4
Feature Type ORF, Verified
Description F-box protein component of an SCF ubiquitin-ligase complex; modular substrate specificity factor which associates with core SCF (Cdc53p, Skp1p and Hrt1p/Rbx1p) to form the SCF(Grr1) complex; SCF(Grr1) acts as a ubiquitin-protein ligase directing ubiquitination of substrates such as: Gic2p, Mks1p, Mth1p, Cln1p, Cln2p and Cln3p; involved in carbon catabolite repression, glucose-dependent divalent cation transport, glucose transport, morphogenesis, and sulfite detoxification (2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
Name Description Glucose Repression-Resistant 1
Chromosomal Location
ChrX:594327 to 590872 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 60 cM
Gene Ontology Annotations All GRR1 GO evidence and references
  View Computational GO annotations for GRR1
Molecular Function
Manually curated
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
Resources
171 total interaction(s) for 88 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 28
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 43
  • Co-localization: 1
  • Co-purification: 1
  • Reconstituted Complex: 9
  • Two-hybrid: 21

Genetic Interactions
  • Dosage Lethality: 7
  • Dosage Rescue: 13
  • Negative Genetic: 1
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 7
  • Synthetic Growth Defect: 11
  • Synthetic Lethality: 14
  • Synthetic Rescue: 11

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,151
Molecular Weight (Da) 132,733
Isoelectric Point (pI) 6.48
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:594327 to 590872 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 60 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3456 594327..590872 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003850
References cited on this page View Complete Literature Guide for GRR1
1) Bailey RB and Woodword A  (1984) Isolation and characterization of a pleiotropic glucose repression resistant mutant of Saccharomyces cerevisiae. Mol Gen Genet 193(3):507-12
2) Conklin DS, et al.  (1993) The COT2 gene is required for glucose-dependent divalent cation transport in Saccharomyces cerevisiae. Mol Cell Biol 13(4):2041-9
3) Avram D and Bakalinsky AT  (1996) Multicopy FZF1 (SUL1) suppresses the sulfite sensitivity but not the glucose derepression or aberrant cell morphology of a grr1 mutant of Saccharomyces cerevisiae. Genetics 144(2):511-21
4) Kishi T, et al.  (1998) Grr1 functions in the ubiquitin pathway in Saccharomyces cerevisiae through association with Skp1. Mol Gen Genet 257(2):143-8
5) Kim YJ, et al.  (1994) Control of cellular morphogenesis by the Ip12/Bem2 GTPase-activating protein: possible role of protein phosphorylation. J Cell Biol 127(5):1381-94
6) Erickson JR and Johnston M  (1994) Suppressors reveal two classes of glucose repression genes in the yeast Saccharomyces cerevisiae. Genetics 136(4):1271-8
7) Vallier LG, et al.  (1994) Altered regulatory responses to glucose are associated with a glucose transport defect in grr1 mutants of Saccharomyces cerevisiae. Genetics 136(4):1279-85
8) Li FN and Johnston M  (1997) Grr1 of Saccharomyces cerevisiae is connected to the ubiquitin proteolysis machinery through Skp1: coupling glucose sensing to gene expression and the cell cycle. EMBO J 16(18):5629-38
9) Barral Y, et al.  (1995) G1 cyclin turnover and nutrient uptake are controlled by a common pathway in yeast. Genes Dev 9(4):399-409
10) Skowyra D, et al.  (1999) Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1. Science 284(5414):662-5
11) Flick JS and Johnston M  (1991) GRR1 of Saccharomyces cerevisiae is required for glucose repression and encodes a protein with leucine-rich repeats. Mol Cell Biol 11(10):5101-12
12) Seol JH, et al.  (1999) Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34. Genes Dev 13(12):1614-26
13) Jaquenoud M, et al.  (1998) The Cdc42p effector Gic2p is targeted for ubiquitin-dependent degradation by the SCFGrr1 complex. EMBO J 17(18):5360-73
14) Berset C, et al.  (2002) Transferable domain in the G(1) cyclin Cln2 sufficient to switch degradation of Sic1 from the E3 ubiquitin ligase SCF(Cdc4) to SCF(Grr1). Mol Cell Biol 22(13):4463-76
15) Liu Z, et al.  (2005) A novel degron-mediated degradation of the RTG pathway regulator, Mks1p, by SCFGrr1. Mol Biol Cell 16(10):4893-904
16) Landry BD, et al.  (2012) F-box protein specificity for g1 cyclins is dictated by subcellular localization. PLoS Genet 8(7):e1002851