FAR1/YJL157C Summary Help

Standard Name FAR1 1
Systematic Name YJL157C
Feature Type ORF, Verified
Description CDK inhibitor and nuclear anchor; during the cell cycle Far1p sequesters the GEF Cdc24p in the nucleus; phosphorylation by Cdc28p-Cln results in SCFCdc4 complex-mediated ubiquitin-dependent degradation, releasing Cdc24p for export and activation of GTPase Cdc42p; in response to pheromone, phosphorylation of Far1p by MAPK Fus3p results in association with, and inhibition of Cdc28p-Cln, as well as Msn5p mediated nuclear export of Far1p-Cdc24p, targeting Cdc24p to polarity sites (1, 2, 3, 4, 5, 6, 7, 8 and see Summary Paragraph)
Name Description Factor ARrest 1
Chromosomal Location
ChrX:126328 to 123836 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All FAR1 GO evidence and references
  View Computational GO annotations for FAR1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulatory Role
Regulatory modules predicted: stressResponse (601, 493, 427)
Mutant phenotypes All FAR1 Phenotype evidence and references
Classical genetics
null
overexpression
repressible
unspecified
Large-scale survey
null
overexpression
Resources
interactions All FAR1 Interaction evidence and references
132 total interaction(s) for 71 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 19
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 16
  • Biochemical Activity: 9
  • Co-crystal Structure: 1
  • Reconstituted Complex: 6
  • Two-hybrid: 18
Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 4
  • Negative Genetic: 20
  • Phenotypic Enhancement: 10
  • Phenotypic Suppression: 15
  • Positive Genetic: 3
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 3
  • Synthetic Rescue: 3
Resources
Expression Summary
histogram
Resources
Protein Information All FAR1 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:126328 to 123836 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..2493 126328..123836 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003693
SUMMARY PARAGRAPH for FAR1

The cyclin dependent kinase inhibitor Far1p is able to inactivate Cdc28p/Clnp complexes, and thus helps stop the cell cycle at START in G1 (2, 9). This is important for mating, in which haploid cells of opposite mating type synchronize their cell cycles by arresting at START, so that they can fuse and become a diploid. Transcription of FAR1 is induced by mating pheromone, in a Ste12p-dependent manner (1). Far1p levels are also controlled by ubiquitin-mediated proteolysis; Far1p is unstable throughout the cell cycle except during G1 (6). Phosphorylation by the MAP kinase homolog Fus3p may help regulate Far1p's association with the Cdc28p/Cln2p complex (7). Far1p may also serve another function in the cell, as part of a Cdc24p-G/beta/gamma-Far1p complex that acts as a landmark for oriented growth during mating (3, 10). This would be made possible by the movement of Far1p from the nucleus to the cytoplasm in response to mating pheromone (10).

Last updated: 1999-09-01

References cited on this page View Complete Literature Guide for FAR1
1) Chang F and Herskowitz I  (1990) Identification of a gene necessary for cell cycle arrest by a negative growth factor of yeast: FAR1 is an inhibitor of a G1 cyclin, CLN2. Cell 63(5):999-1011
2) Peter M and Herskowitz I  (1994) Direct inhibition of the yeast cyclin-dependent kinase Cdc28-Cln by Far1. Science 265(5176):1228-31
3) Nern A and Arkowitz RA  (1999) A Cdc24p-Far1p-Gbetagamma protein complex required for yeast orientation during mating. J Cell Biol 144(6):1187-202
4) Tyers M and Futcher B  (1993) Far1 and Fus3 link the mating pheromone signal transduction pathway to three G1-phase Cdc28 kinase complexes. Mol Cell Biol 13(9):5659-69
5) Shimada Y, et al.  (2000) Nuclear sequestration of the exchange factor Cdc24 by Far1 regulates cell polarity during yeast mating. Nat Cell Biol 2(2):117-24
6) Henchoz S, et al.  (1997) Phosphorylation- and ubiquitin-dependent degradation of the cyclin-dependent kinase inhibitor Far1p in budding yeast. Genes Dev 11(22):3046-60
7) Peter M, et al.  (1993) FAR1 links the signal transduction pathway to the cell cycle machinery in yeast. Cell 73(4):747-60
8) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
9) Mendenhall MD  (1998) Cyclin-dependent kinase inhibitors of Saccharomyces cerevisiae and Schizosaccharomyces pombe. Curr Top Microbiol Immunol 227():1-24
10) Butty AC, et al.  (1998) The role of Far1p in linking the heterotrimeric G protein to polarity establishment proteins during yeast mating. Science 282(5393):1511-6