DAS1/YJL149W Summary Help

Standard Name DAS1 1, 2
Systematic Name YJL149W
Feature Type ORF, Verified
Description Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C (3, 4, 5, 6, 7)
Name Description Dst1-delta 6-Azauracil Sensitivity 2
Chromosomal Location
ChrX:137379 to 139370 | ORF Map | GBrowse
Gene Ontology Annotations All DAS1 GO evidence and references
  View Computational GO annotations for DAS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
Large-scale survey
32 total interaction(s) for 20 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • PCA: 1
  • Reconstituted Complex: 4
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 1
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 2
  • Positive Genetic: 2
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 663
Molecular Weight (Da) 77,366
Isoelectric Point (pI) 6.69
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrX:137379 to 139370 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1992 137379..139370 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003685
References cited on this page View Complete Literature Guide for DAS1
1) Kato M, et al.  (2010) Remodeling of the SCF complex-mediated ubiquitination system by compositional alteration of incorporated F-box proteins. Proteomics 10(1):115-23
2) Gomez-Herreros F, et al.  (2012) TFIIS is required for the balanced expression of the genes encoding ribosomal components under transcriptional stress. Nucleic Acids Res 40(14):6508-19
3) Willems AR, et al.  (1999) SCF ubiquitin protein ligases and phosphorylation-dependent proteolysis. Philos Trans R Soc Lond B Biol Sci 354(1389):1533-50
4) Seol JH, et al.  (2001) Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly. Nat Cell Biol 3(4):384-91
5) Ho Y, et al.  (2002) Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry. Nature 415(6868):180-3
6) Giaever G, et al.  (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91
7) Kus BM, et al.  (2004) Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro. Proteins 54(3):455-67