GCD14/YJL125C Summary Help

Standard Name GCD14 1
Systematic Name YJL125C
Alias TRM61 2
Feature Type ORF, Verified
Description Subunit of tRNA (1-methyladenosine) methyltransferase; required, along with Gcd10p, for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression (3, 4, 5)
Name Description General Control Nonderepressible 6
Gene Product Alias 1-methyladenosine tRNA methyltransferase subunit 3
Chromosomal Location
ChrX:186679 to 185528 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All GCD14 GO evidence and references
  View Computational GO annotations for GCD14
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
reduction of function
repressible
Resources
97 total interaction(s) for 73 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-Western: 2
  • Co-crystal Structure: 1
  • Co-fractionation: 1
  • Co-purification: 3
  • Reconstituted Complex: 1
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Rescue: 5
  • Negative Genetic: 57
  • Phenotypic Enhancement: 12
  • Synthetic Lethality: 3
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 383
Molecular Weight (Da) 43,919
Isoelectric Point (pI) 7.58
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:186679 to 185528 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1152 186679..185528 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003661
References cited on this page View Complete Literature Guide for GCD14
1) Cuesta R, et al.  (1998) Identification of GCD14 and GCD15, novel genes required for translational repression of GCN4 mRNA in Saccharomyces cerevisiae. Genetics 148(3):1007-20
2) Kadaba S, et al.  (2004) Nuclear surveillance and degradation of hypomodified initiator tRNAMet in S. cerevisiae. Genes Dev 18(11):1227-40
3) Anderson J, et al.  (2000) The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine) methyltransferase of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 97(10):5173-8
4) Anderson J, et al.  (1998) The essential Gcd10p-Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA. Genes Dev 12(23):3650-62
5) Hinnebusch A  (1992) "General and Pathway-specific Regulatory Mechanisms Controlling the Synthesis of Amino Acid Biosynthetic Enzymes in Saccharomyces cerevisiae". Pp. 319-414 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
6) Lucchini G, et al.  (1984) Positive regulatory interactions of the HIS4 gene of Saccharomyces cerevisiae. Mol Cell Biol 4(7):1326-33