DPB11/YJL090C Summary Help

Standard Name DPB11 1
Systematic Name YJL090C
Feature Type ORF, Verified
Description DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; along with Rfa1p, binds to ultrafine anaphase bridges in mitotic cells and prevents accumulation of chromatin bridges by stimulating the Mec1p kinase and suppressing homologous recombination; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress (2, 3, 4, 5, 6, 7, 8, 9, 10)
Name Description DNA Polymerase B (II) 1
Chromosomal Location
ChrX:265051 to 262757 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All DPB11 GO evidence and references
  View Computational GO annotations for DPB11
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
conditional
repressible
Large-scale survey
conditional
null
overexpression
reduction of function
repressible
Resources
189 total interaction(s) for 97 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 20
  • Biochemical Activity: 1
  • Protein-peptide: 1
  • Protein-RNA: 1
  • Reconstituted Complex: 14
  • Two-hybrid: 18

Genetic Interactions
  • Dosage Growth Defect: 8
  • Dosage Lethality: 1
  • Dosage Rescue: 13
  • Negative Genetic: 38
  • Phenotypic Enhancement: 17
  • Phenotypic Suppression: 6
  • Positive Genetic: 8
  • Synthetic Growth Defect: 17
  • Synthetic Lethality: 19
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 764
Molecular Weight (Da) 87,240
Isoelectric Point (pI) 9.63
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:265051 to 262757 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2295 265051..262757 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003626
References cited on this page View Complete Literature Guide for DPB11
1) Araki H, et al.  (1995) Dpb11, which interacts with DNA polymerase II(epsilon) in Saccharomyces cerevisiae, has a dual role in S-phase progression and at a cell cycle checkpoint. Proc Natl Acad Sci U S A 92(25):11791-5
2) Masumoto H, et al.  (2000) Dpb11 controls the association between DNA polymerases alpha and epsilon and the autonomously replicating sequence region of budding yeast. Mol Cell Biol 20(8):2809-17
3) Wang H and Elledge SJ  (1999) DRC1, DNA replication and checkpoint protein 1, functions with DPB11 to control DNA replication and the S-phase checkpoint in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 96(7):3824-9
4) Kamimura Y, et al.  (1998) Sld2, which interacts with Dpb11 in Saccharomyces cerevisiae, is required for chromosomal DNA replication. Mol Cell Biol 18(10):6102-9
5) Tanaka S, et al.  (2007) CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast. Nature 445(7125):328-32
6) Navadgi-Patil VM and Burgers PM  (2008) Yeast DNA Replication Protein Dpb11 Activates the Mec1/ATR Checkpoint Kinase. J Biol Chem 283(51):35853-9
7) Mordes DA, et al.  (2008) Dpb11 activates the Mec1-Ddc2 complex. Proc Natl Acad Sci U S A 105(48):18730-4
8) Zegerman P and Diffley JF  (2007) Phosphorylation of Sld2 and Sld3 by cyclin-dependent kinases promotes DNA replication in budding yeast. Nature 445(7125):281-5
9) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
10) Germann SM, et al.  (2014) TopBP1/Dpb11 binds DNA anaphase bridges to prevent genome instability. J Cell Biol 204(1):45-59