YJL068C Summary Help

Systematic Name YJL068C
Feature Type ORF, Verified
Description Esterase that can function as an S-formylglutathione hydrolase; non-essential intracellular esterase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D (1, 2 and see Summary Paragraph)
Also known as: SFGH 3
Chromosomal Location
ChrX:313915 to 313016 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All YJL068C GO evidence and references
  View Computational GO annotations for YJL068C
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
Resources
84 total interaction(s) for 62 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Co-crystal Structure: 1
  • PCA: 2
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 73
  • Positive Genetic: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 299
Molecular Weight (Da) 33,934
Isoelectric Point (pI) 6.7
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:313915 to 313016 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..900 313915..313016 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003604
SUMMARY PARAGRAPH for YJL068C

Yjl068cp is an esterase with S-formylglutathione hydrolase activity which appears to be involved in formaldehyde detoxification (2). Purified Yjl068cp is able to hydroyze a variety of substrates, including S-formylglutathione, carboxyfluorescein diacetate, 4-methylumbelliferyl acetate, p-nitrophenyl acetate, and alpha-naphthyl acetate, but is unable to hydrolyze alpha-naphthyl laurate and alpha-naphthyl oleate (2). YJL068C is induced in response to methyl methanesulfonate (MMS) (4) and neocarzinostatin (NCS), which possesses a regulatory function on the metabolism of DNA and exhibits potent antiproliferative activity in mammalian cells and against gram-positive organisms (5). Null mutants in YJL068C are viable, but display reduced esterase activity and slow growth in the presence of formaldehyde (1, 2). Yjl068cp exhibits similarity to the S-formylglutathione hydrolases of Paracoccus denitrificans, Haemophilus influenzae, Escherichia coli and humans (6, 1).

About glutathione-dependent formaldehyde oxidation

Formaldehyde is formed by oxidative demethylation reactions in many plants and methylotrophic organisms, but Saccharomyces cerevisiae is a nonmethylotrophic yeast and cannot metabolize methanol to formaldehyde. However, S. cerevisiae is exposed to exogenous formaldehyde from plant material or in polluted air and water.

Concentrations of formaldehyde of 1mM or higher are cytostatic or cytotoxic to haploid wild-type cells. Any free formaldehyde in vivo spontaneously reacts with glutathione to form S-hydroxymethylglutathione (7, 8, 2). The level of enzymes involved in the degradation of formaldehyde, such as Sfa1p and Yjl068p, determine the level of formaldehyde toxicity, and cells overproducing Sfa1p are resistant to formaldehyde and null mutants in either sfa1 or yjl068c are hypersensitive to formaldehyde. Sfa1p is induced in response to chemicals such as formaldehyde (FA), ethanol and methyl methanesulphonate, and Yjl068p is also induced in response to chemical stresses (9, 8, 2, 10, 11, 4, 5).

Formate dehydrogenase is encoded by FDH1/YOR388C and FDH2. In some strain backgrounds of S. cerevisiae, FDH2 is encoded by a continuous open reading frame comprised of YPL275W and YPL276W. However, in the systematic sequence of S288C, FDH2 is represented by these two separate open reading frames due to an in frame stop codon (12).

Last updated: 2006-01-24 Contact SGD

References cited on this page View Complete Literature Guide for YJL068C
1) Vandenbol M and Portetelle D  (1999) Disruption of six ORFs on Saccharomyces cerevisiae chromosome X: the YJL069c gene of unknown function is essential to cell viability. Yeast 15(13):1411-7
2) Degrassi G, et al.  (1999) Purification and properties of an esterase from the yeast Saccharomyces cerevisiae and identification of the encoding gene. Appl Environ Microbiol 65(8):3470-2
3) Legler PM, et al.  (2012) A role for His-160 in peroxide inhibition of S. cerevisiae S-formylglutathione hydrolase: evidence for an oxidation sensitive motif. Arch Biochem Biophys 528(1):7-20
4) Jelinsky SA and Samson LD  (1999) Global response of Saccharomyces cerevisiae to an alkylating agent. Proc Natl Acad Sci U S A 96(4):1486-91
5) Schaus SE, et al.  (2001) Gene transcription analysis of Saccharomyces cerevisiae exposed to neocarzinostatin protein-chromophore complex reveals evidence of DNA damage, a potential mechanism of resistance, and consequences of prolonged exposure. Proc Natl Acad Sci U S A 98(20):11075-80
6) Harms N, et al.  (1996) S-formylglutathione hydrolase of Paracoccus denitrificans is homologous to human esterase D: a universal pathway for formaldehyde detoxification? J Bacteriol 178(21):6296-9
7) Achkor H, et al.  (2003) Enhanced formaldehyde detoxification by overexpression of glutathione-dependent formaldehyde dehydrogenase from Arabidopsis. Plant Physiol 132(4):2248-55
8) Grey M, et al.  (1996) Overexpression of ADH1 confers hyper-resistance to formaldehyde in Saccharomyces cerevisiae. Curr Genet 29(5):437-40
9) Wehner E and Brendel M  (1993) Formaldehyde lacks genotoxicity in formaldehyde-hyperresistant strains of the yeast Saccharomyces cerevisiae. Mutat Res 289(1):91-6
10) Wehner EP, et al.  (1993) Molecular structure and genetic regulation of SFA, a gene responsible for resistance to formaldehyde in Saccharomyces cerevisiae, and characterization of its protein product. Mol Gen Genet 237(3):351-8
11) Gompel-Klein P, et al.  (1989) Molecular characterization of the two genes SNQ and SFA that confer hyperresistance to 4-nitroquinoline-N-oxide and formaldehyde in Saccharomyces cerevisiae. Curr Genet 16(2):65-74
12) Overkamp KM, et al.  (2002) Functional analysis of structural genes for NAD(+)-dependent formate dehydrogenase in Saccharomyces cerevisiae. Yeast 19(6):509-20