BET4/YJL031C Summary Help

Standard Name BET4 1
Systematic Name YJL031C
Feature Type ORF, Verified
Description Alpha subunit of Type II geranylgeranyltransferase; required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p (2 and see Summary Paragraph)
Name Description Blocked Early in Transport 3
Chromosomal Location
ChrX:387438 to 386368 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All BET4 GO evidence and references
  View Computational GO annotations for BET4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Classical genetics
Large-scale survey
reduction of function
18 total interaction(s) for 13 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 3
  • Co-purification: 1
  • FRET: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Rescue: 1
  • Negative Genetic: 1
  • Positive Genetic: 1
  • Synthetic Lethality: 2

Expression Summary
Length (a.a.) 327
Molecular Weight (Da) 39,674
Isoelectric Point (pI) 7.26
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrX:387438 to 386368 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 2004-01-27
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3 387438..387436 2011-02-03 2004-01-27
Intron 4..90 387435..387349 2011-02-03 2004-01-27
CDS 91..1071 387348..386368 2011-02-03 2004-01-27
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003568

Bet4p, the alpha subunit of the type II (RAB-protein) geranylgeranyl transferase (GGTase II) (4, 5) forms the catalytic component (component B) along with Bet2p. Bet2p and Bet4p form a stable heterodimer and Mrs6p, the accessory subunit, presents the substrate to these catalytic subunits. GGTase II (Bet2p, Bet4p, Mrs6p) is required for transport between the endoplasmic reticulum and the Golgi, and its role in that pathway seems to be in geranylgeranylating (prenylating) Ypt1p and Sec4p, small GTP-binding proteins that help mediate vesicular traffic. Blocking of prenylation leads to defects in the membrane attachment of Ypt1 and Sec4p (6, 7, 8).
Note: The genetically-defined MAD2 gene was originally identified as YJL031C (see Li R, et al. (1993) Nature 366(6450):82-4, and erratum in Nature (1994) 371(6496):438). It is now known that MAD2, involved in the mitotic spindle checkpoint, is equivalent to YJL030W, while YJL031C/BET4 encodes the alpha subunit of geranylgeranyltransferase type II.

The type II GGTase is one of three prenyl transferases in yeast, the other two being farnesyl transferase and the type I GGTase (8, 5).

Last updated: 2010-05-18 Contact SGD

References cited on this page View Complete Literature Guide for BET4
1) Jiang Y, et al.  (1995) BTS1 encodes a geranylgeranyl diphosphate synthase in Saccharomyces cerevisiae. J Biol Chem 270(37):21793-9
2) Witter DJ and Poulter CD  (1996) Yeast geranylgeranyltransferase type-II: steady state kinetic studies of the recombinant enzyme. Biochemistry 35(32):10454-63
3) Newman AP and Ferro-Novick S  (1987) Characterization of new mutants in the early part of the yeast secretory pathway isolated by a [3H]mannose suicide selection. J Cell Biol 105(4):1587-94
4) Rossi G, et al.  (1991) Dependence of Ypt1 and Sec4 membrane attachment on Bet2. Nature 351(6322):158-61
5) Jiang Y, et al.  (1993) Bet2p and Mad2p are components of a prenyltransferase that adds geranylgeranyl onto Ypt1p and Sec4p. Nature 366(6450):84-6
6) Benito-Moreno RM, et al.  (1994) Mrs6p, the yeast homologue of the mammalian choroideraemia protein: immunological evidence for its function as the Ypt1p Rab escort protein. Curr Genet 27(1):23-5
7) Jiang Y and Ferro-Novick S  (1994) Identification of yeast component A: reconstitution of the geranylgeranyltransferase that modifies Ypt1p and Sec4p. Proc Natl Acad Sci U S A 91(10):4377-81
8) Jiang Y, et al.  (1995) Characterization of yeast type-II geranylgeranyltransferase. Methods Enzymol 257:21-9