MAD2/YJL030W Summary Help

Standard Name MAD2 1
Systematic Name YJL030W
Feature Type ORF, Verified
Description Component of the spindle-assembly checkpoint complex; delays onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability (1, 2, 3 and see Summary Paragraph)
Name Description Mitotic Arrest-Deficient 1
Chromosomal Location
ChrX:387657 to 388247 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All MAD2 GO evidence and references
  View Computational GO annotations for MAD2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
overexpression
unspecified
Large-scale survey
null
overexpression
Resources
522 total interaction(s) for 245 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 13
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 14
  • Biochemical Activity: 5
  • Co-fractionation: 2
  • Co-purification: 3
  • PCA: 2
  • Reconstituted Complex: 4
  • Two-hybrid: 24

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 3
  • Dosage Rescue: 2
  • Negative Genetic: 119
  • Phenotypic Enhancement: 24
  • Phenotypic Suppression: 39
  • Positive Genetic: 2
  • Synthetic Growth Defect: 94
  • Synthetic Lethality: 148
  • Synthetic Rescue: 19

Resources
Expression Summary
histogram
Resources
Length (a.a.) 196
Molecular Weight (Da) 22,284
Isoelectric Point (pI) 5.15
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:387657 to 388247 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..591 387657..388247 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003567
SUMMARY PARAGRAPH for MAD2

MAD2 is a non-essential gene that encodes a component of the spindle checkpoint (1, 4). The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules (see 5 for review; reference 6 describes defects detected by the checkpoint). The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators (5). Like other spindle checkpoint mutants, mad2 loss-of-function mutants are sensitive to benomyl and cannot delay cell division in response to spindle depolymerization (1). Mad2p forms a tight complex with another spindle checkpoint protein, Mad1p, throughout the cell cycle (7). Mad2p also forms a complex with Cdc20p, which activates the anaphase promoting complex, and Mad3p; the presence of Mad1p is required for the complex to form (8). Bub1p, Bub3p, Mad1p, Mad2p, Mad3p, and the protein kinase Mps1p act in a branch of the spindle checkpoint pathway that may prevent premature chromosome disjunction. A second branch involves Bub2p and Bfa1p, and may prevent cytokinesis prior to chromosome segregation (9, 10, 11, 12). Homologs of Mad2p act in the spindle checkpoint in Xenopus (Xmad2; 13, 14) and human (MAD2L1 and MAD2L2; 15, 16). MAD2 was originally identified as the open reading frame YJL031C (see 17 and erratum in Nature (1994) 371(6496):438). However, it is now known that MAD2 corresponds to the adjacent open reading frame YJL030W, while YJL031C encodes the geranylgeranyltransferase subunit Bet4p.

Last updated: 2005-07-01 Contact SGD

References cited on this page View Complete Literature Guide for MAD2
1) Li R and Murray AW  (1991) Feedback control of mitosis in budding yeast. Cell 66(3):519-31
2) Tsuchiya D, et al.  (2011) The spindle checkpoint protein Mad2 regulates APC/C activity during prometaphase and metaphase of meiosis I in Saccharomyces cerevisiae. Mol Biol Cell 22(16):2848-61
3) Zhu J, et al.  (2012) Karyotypic determinants of chromosome instability in aneuploid budding yeast. PLoS Genet 8(5):e1002719
4) Hardwick KG and Murray AW  (1995) Mad1p, a phosphoprotein component of the spindle assembly checkpoint in budding yeast. J Cell Biol 131(3):709-20
5) Hardwick KG  (1998) The spindle checkpoint. Trends Genet 14(1):1-4
6) Hardwick KG, et al.  (1999) Lesions in many different spindle components activate the spindle checkpoint in the budding yeast Saccharomyces cerevisiae. Genetics 152(2):509-18
7) Chen RH, et al.  (1999) The spindle checkpoint of budding yeast depends on a tight complex between the Mad1 and Mad2 proteins. Mol Biol Cell 10(8):2607-18
8) Hwang LH, et al.  (1998) Budding yeast Cdc20: a target of the spindle checkpoint. Science 279(5353):1041-4
9) Alexandru G, et al.  (1999) Sister chromatid separation and chromosome re-duplication are regulated by different mechanisms in response to spindle damage. EMBO J 18(10):2707-21
10) Fesquet D, et al.  (1999) A Bub2p-dependent spindle checkpoint pathway regulates the Dbf2p kinase in budding yeast. EMBO J 18(9):2424-34
11) Fraschini R, et al.  (1999) Budding yeast Bub2 is localized at spindle pole bodies and activates the mitotic checkpoint via a different pathway from Mad2. J Cell Biol 145(5):979-91
12) Li R  (1999) Bifurcation of the mitotic checkpoint pathway in budding yeast. Proc Natl Acad Sci U S A 96(9):4989-94
13) Chen RH, et al.  (1996) Association of spindle assembly checkpoint component XMAD2 with unattached kinetochores. Science 274(5285):242-6
14) Chen RH, et al.  (1998) Spindle checkpoint protein Xmad1 recruits Xmad2 to unattached kinetochores. J Cell Biol 143(2):283-95
15) Li Y and Benezra R  (1996) Identification of a human mitotic checkpoint gene: hsMAD2. Science 274(5285):246-8
16) Cahill DP, et al.  (1999) Characterization of MAD2B and other mitotic spindle checkpoint genes. Genomics 58(2):181-7
17) Li R, et al.  (1993) The mitotic feedback control gene MAD2 encodes the alpha-subunit of a prenyltransferase. Nature 366(6450):82-4