VTC4/YJL012C Summary Help

Standard Name VTC4 1
Systematic Name YJL012C
Alias PHM3 2 , YJL012C-A
Feature Type ORF, Verified
Description Vacuolar membrane polyphosphate polymerase; subunit of the vacuolar transporter chaperone (VTC) complex involved in synthesis and transfer of polyP to the vacuole; regulates membrane trafficking; role in non-autophagic vacuolar fusion; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5, 6, 7)
Name Description Vacuolar Transporter Chaperone 1
Chromosomal Location
ChrX:413399 to 411234 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All VTC4 GO evidence and references
  View Computational GO annotations for VTC4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 9 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
61 total interaction(s) for 42 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 11
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 4
  • Co-purification: 1
  • PCA: 8
  • Reconstituted Complex: 1
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 7
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 12
  • Positive Genetic: 4
  • Synthetic Growth Defect: 4
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 721
Molecular Weight (Da) 83,154
Isoelectric Point (pI) 6.81
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:413399 to 411234 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
This feature contains embedded feature(s): YJL012C-A
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2004-02-19
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2166 413399..411234 2011-02-03 2004-02-19
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003549
References cited on this page View Complete Literature Guide for VTC4
1) Cohen A, et al.  (1999) A novel family of yeast chaperons involved in the distribution of V-ATPase and other membrane proteins. J Biol Chem 274(38):26885-93
2) Ogawa N, et al.  (2000) New components of a system for phosphate accumulation and polyphosphate metabolism in Saccharomyces cerevisiae revealed by genomic expression analysis. Mol Biol Cell 11(12):4309-21
3) Muller O, et al.  (2002) The Vtc proteins in vacuole fusion: coupling NSF activity to V(0) trans-complex formation. EMBO J 21(3):259-69
4) Muller O, et al.  (2003) Role of the Vtc proteins in V-ATPase stability and membrane trafficking. J Cell Sci 116(Pt 6):1107-15
5) Uttenweiler A, et al.  (2007) The vacuolar transporter chaperone (VTC) complex is required for microautophagy. Mol Biol Cell 18(1):166-75
6) Hothorn M, et al.  (2009) Catalytic core of a membrane-associated eukaryotic polyphosphate polymerase. Science 324(5926):513-6
7) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76