IST3/YIR005W Summary Help

Standard Name IST3 1
Systematic Name YIR005W
Alias SNU17 2
Feature Type ORF, Verified
Description Component of the U2 snRNP; required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing; diploid mutants have a specific defect in MATa1 pre-mRNA splicing which leads to haploid gene expression in diploids (2, 3, 4, 5)
Name Description Increased Sodium Tolerance 1
Chromosomal Location
ChrIX:364889 to 365335 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All IST3 GO evidence and references
  View Computational GO annotations for IST3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 70 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
323 total interaction(s) for 197 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 22
  • Co-purification: 1
  • Reconstituted Complex: 7
  • Two-hybrid: 5

Genetic Interactions
  • Negative Genetic: 193
  • Positive Genetic: 59
  • Synthetic Growth Defect: 24
  • Synthetic Lethality: 4
  • Synthetic Rescue: 8

Resources
Expression Summary
histogram
Resources
Length (a.a.) 148
Molecular Weight (Da) 17,098
Isoelectric Point (pI) 5.79
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:364889 to 365335 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..447 364889..365335 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001444
References cited on this page View Complete Literature Guide for IST3
1) Entian KD, et al.  (1999) Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approach. Mol Gen Genet 262(4-5):683-702
2) Gottschalk A, et al.  (2001) A novel yeast U2 snRNP protein, Snu17p, is required for the first catalytic step of splicing and for progression of spliceosome assembly. Mol Cell Biol 21(9):3037-46
3) Wang Q and Rymond BC  (2003) Rds3p is required for stable U2 snRNP recruitment to the splicing apparatus. Mol Cell Biol 23(20):7339-49
4) Spingola M, et al.  (2004) Mer1p is a modular splicing factor whose function depends on the conserved U2 snRNP protein Snu17p. Nucleic Acids Res 32(3):1242-50
5) Tuo S, et al.  (2012) Apparent defect in yeast bud-site selection due to a specific failure to splice the pre-mRNA of a regulator of cell-type-specific transcription. PLoS One 7(10):e47621