Standard Name	TID3
Systematic Name	YIL144W
Alias	NDC80 , HEC1
Feature Type	ORF, Verified
Description	Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering (1, 2, 3, 4, 5, 6)
Name Description	Two-hybrid Interaction with Dmc1p 7

Chromosomal Location	ChrIX:78074 to 80149 | ORF Map | GBrowse

	View Computational GO annotations for TID3
Molecular Function
Manually curated	structural constituent of cytoskeleton (IPI)
Biological Process
Manually curated	chromosome segregation (IGI, IMP) microtubule nucleation (IPI) protein localization to kinetochore (IMP)
Cellular Component
Manually curated	condensed nuclear chromosome kinetochore (IDA, IMP, IPI) condensed nuclear chromosome, centromeric region (IDA, IGI, IPI) Ndc80 complex (IDA, IPI)

Classical genetics
overexpression	budding index: abnormal cell cycle progression in G2 phase: abnormal cell size: increased nuclear morphology: abnormal
reduction of function	resistance to benomyl: decreased
unspecified	cell cycle progression in M phase: delayed chromosome/plasmid maintenance: abnormal
Large-scale survey
conditional	colony sectoring: increased
null	inviable
overexpression	vegetative growth: decreased
reduction of function	competitive fitness: increased telomere length: decreased
repressible	chromosome/plasmid maintenance: decreased
Resources	PROPHECY | PhenoM | SCMD | ScreenTroll | Yeast Fitness Database

	185 total interaction(s) for 92 unique genes/features.
Physical Interactions	Affinity Capture-MS: 36 Affinity Capture-Western: 32 Biochemical Activity: 2 Co-fractionation: 3 Co-localization: 3 Co-purification: 8 PCA: 2 Reconstituted Complex: 7 Two-hybrid: 72
Genetic Interactions	Dosage Rescue: 5 Phenotypic Enhancement: 2 Phenotypic Suppression: 1 Positive Genetic: 1 Synthetic Growth Defect: 1 Synthetic Lethality: 7 Synthetic Rescue: 3
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Mass Spec

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIX:78074 to 80149 | ORF Map | GBrowse
Last Update	Coordinates: 1994-12-10 | Sequence: 1994-12-10
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2076 78074..80149 1994-12-10 1994-12-10
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001406