MET18/YIL128W Summary Help

Standard Name MET18 1
Systematic Name YIL128W
Alias MMS19 2
Feature Type ORF, Verified
Description Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19 (3, 4, 5)
Name Description METhionine requiring 1
Chromosomal Location
ChrIX:113806 to 116904 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All MET18 GO evidence and references
  View Computational GO annotations for MET18
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 5 genes
Resources
Classical genetics
null
reduction of function
unspecified
Large-scale survey
null
Resources
267 total interaction(s) for 237 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 28
  • Affinity Capture-Western: 10
  • PCA: 1
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 183
  • Phenotypic Suppression: 4
  • Positive Genetic: 21
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 8
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,032
Molecular Weight (Da) 117,881
Isoelectric Point (pI) 5.72
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:113806 to 116904 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 1994-12-10 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3099 113806..116904 1994-12-10 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001390
References cited on this page View Complete Literature Guide for MET18
1) Masselot M and De Robichon-Szulmajster H  (1975) Methionine biosynthesis in Saccharomyces cerevisiae. I. Genetical analysis of auxotrophic mutants. Mol Gen Genet 139(2):121-32
2) Prakash L and Prakash S  (1977) Isolation and characterization of MMS-sensitive mutants of Saccharomyces cerevisiae. Genetics 86(1):33-55
3) Queimado L, et al.  (2001) Cloning the human and mouse MMS19 genes and functional complementation of a yeast mms19 deletion mutant. Nucleic Acids Res 29(9):1884-91
4) Stehling O, et al.  (2012) MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity. Science 337(6091):195-9
5) Gari K, et al.  (2012) MMS19 links cytoplasmic iron-sulfur cluster assembly to DNA metabolism. Science 337(6091):243-5