Standard Name	MET18 1
Systematic Name	YIL128W
Alias	MMS19 2
Feature Type	ORF, Verified
Description	Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19 (3, 4, 5)
Name Description	METhionine requiring 1

Chromosomal Location	ChrIX:113806 to 116904 | ORF Map | GBrowse

	View Computational GO annotations for MET18
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	iron-sulfur cluster assembly (IMP)
Cellular Component
High-throughput	cytoplasm (IDA)

Regulatory modules	predicted: stressResponse (475) predicted: cellcycle (389) predicted: stressResponse (475) predicted: cellcycle (389)
Resources	YeTFaSCo

Classical genetics
null	auxotrophy resistance to 6-azauracil: increased resistance to mycophenolic acid: increased resistance to hydroxyurea: decreased
reduction of function	UV resistance: decreased X ray resistance: normal resistance to methyl methanesulfonate: decreased
unspecified	resistance to sulfite: decreased
Large-scale survey
null	competitive fitness: decreased endocytosis: decreased oxidative stress resistance: decreased Fus-Mid-GFP distribution: abnormal resistance to methyl methanesulfonate: decreased resistance to hydroxyurea: decreased resistance to amiodarone: decreased resistance to sulfanilamide: decreased resistance to oleate: decreased resistance to tunicamycin: increased resistance to mycophenolic acid: increased resistance to bleomycin: increased resistance to 1,4-dithiothreitol and tacrolimus (anhydrous): increased resistance to tacrolimus (anhydrous) and tunicamycin: increased resistance to sodium arsenite: increased resistance to farnesol: decreased resistance to CTBT: decreased resistance to ethanol: decreased resistance to (S)-lactic acid: decreased resistance to sodium selenide: decreased resistance to fenpropimorph: increased resistance to sorbate: decreased respiratory growth: decreased rate starvation resistance: decreased telomere length: increased utilization of nitrogen source: absent vacuolar morphology: abnormal viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	264 total interaction(s) for 236 unique genes/features.
Physical Interactions	Affinity Capture-MS: 27 Affinity Capture-Western: 9 PCA: 1 Two-hybrid: 2
Genetic Interactions	Negative Genetic: 182 Phenotypic Suppression: 4 Positive Genetic: 21 Synthetic Growth Defect: 9 Synthetic Lethality: 8 Synthetic Rescue: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIX:113806 to 116904 | ORF Map | GBrowse
Last Update	Coordinates: 1994-12-10 | Sequence: 1994-12-10
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..3099 113806..116904 1994-12-10 1994-12-10
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001390