MET30/YIL046W Summary Help

Standard Name MET30 1
Systematic Name YIL046W
Alias ZRG11 2
Feature Type ORF, Verified
Description F-box protein containing five copies of the WD40 motif; controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus; dissociation of Met30p from SCF complex in response to cadmium stress is regulated by Cdc48p (3, 4, 5)
Name Description METhionine requiring
Chromosomal Location
ChrIX:268651 to 270573 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All MET30 GO evidence and references
  View Computational GO annotations for MET30
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Resources
Classical genetics
conditional
null
overexpression
Large-scale survey
null
reduction of function
repressible
Resources
178 total interaction(s) for 108 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 20
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 27
  • Co-localization: 3
  • Co-purification: 1
  • PCA: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 18

Genetic Interactions
  • Dosage Lethality: 6
  • Dosage Rescue: 6
  • Negative Genetic: 68
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 1
  • Synthetic Growth Defect: 4
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 8
  • Synthetic Rescue: 9

Resources
Expression Summary
histogram
Resources
Length (a.a.) 640
Molecular Weight (Da) 72,835
Isoelectric Point (pI) 7.56
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:268651 to 270573 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1923 268651..270573 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001308
References cited on this page View Complete Literature Guide for MET30
1) Thomas D, et al.  (1995) Met30p, a yeast transcriptional inhibitor that responds to S-adenosylmethionine, is an essential protein with WD40 repeats. Mol Cell Biol 15(12):6526-34
2) Yuan DS  (2000) Zinc-regulated genes in Saccharomyces cerevisiae revealed by transposon tagging. Genetics 156(1):45-58
3) Rouillon A, et al.  (2000) Feedback-regulated degradation of the transcriptional activator Met4 is triggered by the SCF(Met30 )complex. EMBO J 19(2):282-94
4) Smothers DB, et al.  (2000) The abundance of Met30p limits SCF(Met30p) complex activity and is regulated by methionine availability. Mol Cell Biol 20(21):7845-52
5) Yen JL, et al.  (2012) Signal-induced disassembly of the SCF ubiquitin ligase complex by Cdc48/p97. Mol Cell 48(2):288-97