DNA2/YHR164C Summary Help

Standard Name DNA2 1
Systematic Name YHR164C
Alias WEB2
Feature Type ORF, Verified
Description Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; tracking protein for flap cleavage during Okazaki fragment maturation; involved in DNA repair and processing of meiotic DNA double strand breaks; required for normal life span; component of telomeric chromatin, with cell-cycle dependent localization; required for telomerase-dependent telomere synthesis; forms nuclear foci upon DNA replication stress (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
Name Description DNA synthesis defective 1
Chromosomal Location
ChrVIII:429178 to 424610 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All DNA2 GO evidence and references
  View Computational GO annotations for DNA2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 1 genes
Resources
Classical genetics
conditional
overexpression
reduction of function
repressible
unspecified
Large-scale survey
conditional
null
overexpression
reduction of function
repressible
Resources
139 total interaction(s) for 76 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 17
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 7
  • Biochemical Activity: 1
  • Co-localization: 2
  • Reconstituted Complex: 2
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 16
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 5
  • Positive Genetic: 3
  • Synthetic Growth Defect: 20
  • Synthetic Lethality: 41
  • Synthetic Rescue: 16

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,522
Molecular Weight (Da) 171,693
Isoelectric Point (pI) 6.43
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:429178 to 424610 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..4569 429178..424610 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001207
References cited on this page View Complete Literature Guide for DNA2
1) Dumas LB, et al.  (1982) New temperature-sensitive mutants of Saccharomyces cerevisiae affecting DNA replication. Mol Gen Genet 187(1):42-6
2) Budd ME and Campbell JL  (2000) The pattern of sensitivity of yeast dna2 mutants to DNA damaging agents suggests a role in DSB and postreplication repair pathways. Mutat Res 459(3):173-86
3) Budd ME and Campbell JL  (1997) A yeast replicative helicase, Dna2 helicase, interacts with yeast FEN-1 nuclease in carrying out its essential function. Mol Cell Biol 17(4):2136-42
4) Schlesinger MB and Formosa T  (2000) POB3 is required for both transcription and replication in the yeast Saccharomyces cerevisiae. Genetics 155(4):1593-606
5) Bae SH, et al.  (1998) Dna2 of Saccharomyces cerevisiae possesses a single-stranded DNA-specific endonuclease activity that is able to act on double-stranded DNA in the presence of ATP. J Biol Chem 273(41):26880-90
6) Bae SH and Seo YS  (2000) Characterization of the enzymatic properties of the yeast dna2 Helicase/endonuclease suggests a new model for Okazaki fragment processing. J Biol Chem 275(48):38022-31
7) Hoopes LL, et al.  (2002) Mutations in DNA replication genes reduce yeast life span. Mol Cell Biol 22(12):4136-46
8) Choe W, et al.  (2002) Dynamic localization of an Okazaki fragment processing protein suggests a novel role in telomere replication. Mol Cell Biol 22(12):4202-17
9) Kao HI, et al.  (2004) Dna2p helicase/nuclease is a tracking protein, like FEN1, for flap cleavage during Okazaki fragment maturation. J Biol Chem 279(49):50840-9
10) Kosugi S, et al.  (2009) Systematic identification of cell cycle-dependent yeast nucleocytoplasmic shuttling proteins by prediction of composite motifs. Proc Natl Acad Sci U S A 106(25):10171-6
11) Manfrini N, et al.  (2010) Processing of meiotic DNA double strand breaks requires cyclin-dependent kinase and multiple nucleases. J Biol Chem 285(15):11628-37
12) Balakrishnan L, et al.  (2010) Dna2 exhibits a unique strand end-dependent helicase function. J Biol Chem 285(50):38861-8
13) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76