KSP1/YHR082C Summary Help

Standard Name KSP1
Systematic Name YHR082C
Feature Type ORF, Verified
Description Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5)
Chromosomal Location
ChrVIII:271548 to 268459 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All KSP1 GO evidence and references
  View Computational GO annotations for KSP1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
High-throughput
Regulators 6 genes
Resources
Classical genetics
null
reduction of function
unspecified
Large-scale survey
null
overexpression
unspecified
Resources
357 total interaction(s) for 337 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 31
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 2
  • Biochemical Activity: 190
  • PCA: 1
  • Protein-RNA: 1
  • Reconstituted Complex: 10
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 13
  • Dosage Lethality: 3
  • Dosage Rescue: 1
  • Negative Genetic: 86
  • Phenotypic Suppression: 2
  • Positive Genetic: 9
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,029
Molecular Weight (Da) 117,081
Isoelectric Point (pI) 6.56
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:271548 to 268459 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3090 271548..268459 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001124
References cited on this page View Complete Literature Guide for KSP1
1) Fleischmann M, et al.  (1996) Allele-specific suppression of a Saccharomyces cerevisiae prp20 mutation by overexpression of a nuclear serine/threonine protein kinase. Mol Gen Genet 250(5):614-25
2) Bharucha N, et al.  (2008) Analysis of the Yeast Kinome Reveals a Network of Regulated Protein Localization during Filamentous Growth. Mol Biol Cell 19(7):2708-17
3) Laxman S and Tu BP  (2011) Multiple TORC1-Associated Proteins Regulate Nitrogen Starvation-Dependent Cellular Differentiation in Saccharomyces cerevisiae. PLoS One 6(10):e26081
4) Umekawa M and Klionsky DJ  (2012) Ksp1 kinase regulates autophagy via the target of rapamycin complex 1 (TORC1) pathway. J Biol Chem 287(20):16300-10
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76