Standard Name	VMA22
Systematic Name	YHR060W
Alias	CEV1 1 , VPH6
Feature Type	ORF, Verified
Description	Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) (2, 3, 4)

Chromosomal Location	ChrVIII:220725 to 221270 | ORF Map | GBrowse

Molecular Function
Manually curated	unfolded protein binding (IGI, IMP, IPI)
Biological Process
Manually curated	vacuolar acidification (IMP) vacuolar proton-transporting V-type ATPase complex assembly (IMP)
Cellular Component
Manually curated	extrinsic to endoplasmic reticulum membrane (IDA, IMP, IPI)

Classical genetics
null	metal resistance: decreased Fbp1p accumulation: increased resistance to propolis extract: decreased resistance to edelfosine: decreased vacuolar morphology: abnormal
Large-scale survey
null	acid pH resistance: decreased alkaline pH resistance: decreased auxotrophy cell cycle progression in G1 phase: increased duration arginine accumulation: decreased proton accumulation: decreased polyphosphate accumulation: abnormal glycogen accumulation: increased heat sensitivity: increased lipid particle morphology: abnormal metal resistance: decreased mitochondrial morphology: abnormal oxidative stress resistance: decreased Cps1p (CPS) modification: decreased Pho8p (ALP) modification: decreased resistance to cycloheximide: decreased resistance to calcium dichloride: decreased resistance to ethanol: decreased resistance to CTBT: decreased resistance to dihydromotuporamine C: decreased resistance to propan-1-ol: decreased resistance to sulfanilamide: decreased resistance to oxalic acid: decreased resistance to citric acid: decreased resistance to L-1,4-dithiothreitol: decreased resistance to cyclosporin A: decreased resistance to FK506: decreased resistance to tunicamycin: decreased resistance to propolis extract: decreased resistance to pyrrolidine dithiocarbamate: decreased resistance to quinine: decreased resistance to hygromycin B: decreased resistance to spermine: decreased resistance to boric acid: decreased resistance to chloroquine: decreased resistance to ciclopirox olamine: decreased resistance to curcumin: decreased resistance to hydroxyurea: decreased resistance to caffeine: decreased resistance to doxorubicin: decreased respiratory growth: absent toxin resistance: increased utilization of carbon source: decreased utilization of iron source: decreased vegetative growth: decreased rate viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	56 total interaction(s) for 50 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Affinity Capture-RNA: 3 Affinity Capture-Western: 2 Biochemical Activity: 1 PCA: 2 Two-hybrid: 8
Genetic Interactions	Negative Genetic: 20 Synthetic Growth Defect: 13 Synthetic Lethality: 6
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrVIII:220725 to 221270 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..546 220725..221270 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001102