AAP1/YHR047C Summary Help

Standard Name AAP1 1
Systematic Name YHR047C
Alias AAP1'
Feature Type ORF, Verified
Description Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication (1, 2 and see Summary Paragraph)
Name Description Arginine/alanine AminoPeptidase 1
Chromosomal Location
ChrVIII:201310 to 198740 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All AAP1 GO evidence and references
  View Computational GO annotations for AAP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Regulators 5 genes
Large-scale survey
24 total interaction(s) for 17 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 1
  • Co-purification: 1

Genetic Interactions
  • Negative Genetic: 12

Expression Summary
Length (a.a.) 856
Molecular Weight (Da) 97,662
Isoelectric Point (pI) 5.01
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVIII:201310 to 198740 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2571 201310..198740 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001089

Aap1p is an alanine/arginine aminopeptidase that localizes to the nucleus and cytoplasm (1, 3). Aap1p acts as a positive regulator of glycogen accumulation, either directly or indirectly (1). AAP1 is constitutively expressed under normal growth conditions (1), and is induced in a Gcn4p-dependent manner in response to histidine starvation (4). AAP1 expression is decreased in an mpk1 null mutant relative to wild-type in response to linoleic acid hydroperoxide (LoaOOH) (5).

aap1 null mutants are viable, but accumulate less glycogen than wild type at the diauxic shift when glucose is depleted from the medium, and display a slight dampening of the heat shock response (1). Overproduction of Aap1p results in hyperaccumulation of glycogen, an increased growth rate on acetate-based medium, and increased expression of the HSP70-related SSA3 gene during entry into stationary phase. Overproduction also suppresses the temperature-sensitive growth defect of an spr5-27 mutant on non-fermentable carbon sources (1).

Aap1p is a member of the M1 family of metalloaminopeptidases, which includes Ape2p and bacterial and mammalian membrane alanyl aminopeptidases, mammalian glutamyl aminopeptidases and leukotriene A-4 hydrolases (human LTA4H). Aap1p also has similarity to Aspergillus niger ApsA and to mouse pre-B cell antigen BPI (1, 6).

Last updated: 2006-04-05 Contact SGD

References cited on this page View Complete Literature Guide for AAP1
1) Caprioglio DR, et al.  (1993) Isolation and characterization of AAP1. A gene encoding an alanine/arginine aminopeptidase in yeast. J Biol Chem 268(19):14310-5
2) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
3) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
4) Natarajan K, et al.  (2001) Transcriptional profiling shows that Gcn4p is a master regulator of gene expression during amino acid starvation in yeast. Mol Cell Biol 21(13):4347-68
5) Alic N, et al.  (2003) Lipid hydroperoxides activate the mitogen-activated protein kinase Mpk1p in Saccharomyces cerevisiae. J Biol Chem 278(43):41849-55
6) Hinnebusch AG and Natarajan K  (2002) Gcn4p, a master regulator of gene expression, is controlled at multiple levels by diverse signals of starvation and stress. Eukaryot Cell 1(1):22-32