PIH1/YHR034C Summary Help

Standard Name PIH1 1
Systematic Name YHR034C
Alias NOP17 2
Feature Type ORF, Verified
Description Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II (1, 2, 3, 4, 5, 6)
Name Description Protein Interacting with Hsp90 1
Chromosomal Location
ChrVIII:177999 to 176965 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PIH1 GO evidence and references
  View Computational GO annotations for PIH1
Molecular Function
Manually curated
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
High-throughput
Regulators 1 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
157 total interaction(s) for 99 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 12
  • Co-crystal Structure: 1
  • Co-fractionation: 1
  • PCA: 1
  • Reconstituted Complex: 7
  • Two-hybrid: 23

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 57
  • Positive Genetic: 26
  • Synthetic Growth Defect: 16
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 344
Molecular Weight (Da) 39,519
Isoelectric Point (pI) 5.76
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:177999 to 176965 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1035 177999..176965 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001076
References cited on this page View Complete Literature Guide for PIH1
1) Zhao R, et al.  (2005) Navigating the chaperone network: an integrative map of physical and genetic interactions mediated by the hsp90 chaperone. Cell 120(5):715-27
2) Gonzales FA, et al.  (2005) Characterization of Saccharomyces cerevisiae Nop17p, a novel Nop58p-interacting protein that is involved in Pre-rRNA processing. J Mol Biol 346(2):437-55
3) Jonsson ZO, et al.  (2004) Rvb1p/Rvb2p recruit Arp5p and assemble a functional Ino80 chromatin remodeling complex. Mol Cell 16(3):465-77
4) Eckert K, et al.  (2010) The Pih1-Tah1 cochaperone complex inhibits Hsp90 molecular chaperone ATPase activity. J Biol Chem 285(41):31304-12
5) Zhao R, et al.  (2008) Molecular chaperone Hsp90 stabilizes Pih1/Nop17 to maintain R2TP complex activity that regulates snoRNA accumulation. J Cell Biol 180(3):563-78
6) Jimenez B, et al.  (2012) Structure of minimal tetratricopeptide repeat domain protein Tah1 reveals mechanism of its interaction with Pih1 and Hsp90. J Biol Chem 287(8):5698-709