NPR3/YHL023C Summary Help

Standard Name NPR3 1
Systematic Name YHL023C
Alias RMD11 2
Feature Type ORF, Verified
Description Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair (1, 2, 3, 4, 5)
Name Description Nitrogen Permease Regulator 1
Chromosomal Location
ChrVIII:62563 to 59123 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All NPR3 GO evidence and references
  View Computational GO annotations for NPR3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
115 total interaction(s) for 103 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 16
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 5

Genetic Interactions
  • Negative Genetic: 69
  • Phenotypic Enhancement: 2
  • Positive Genetic: 12
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,146
Molecular Weight (Da) 129,972
Isoelectric Point (pI) 6.34
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:62563 to 59123 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3441 62563..59123 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001015
References cited on this page View Complete Literature Guide for NPR3
1) Neklesa TK and Davis RW  (2009) A Genome-Wide Screen for Regulators of TORC1 in Response to Amino Acid Starvation Reveals a Conserved Npr2/3 Complex. PLoS Genet 5(6):e1000515
2) Enyenihi AH and Saunders WS  (2003) Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae. Genetics 163(1):47-54
3) Jordan PW, et al.  (2007) Novel roles for selected genes in meiotic DNA processing. PLoS Genet 3(12):e222
4) Dokudovskaya S, et al.  (2011) A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae. Mol Cell Proteomics 10(6):M110.006478
5) Wu X and Tu BP  (2011) Selective regulation of autophagy by the Iml1-Npr2-Npr3 complex in the absence of nitrogen starvation. Mol Biol Cell 22(21):4124-33