SPT6/YGR116W Summary Help

Standard Name SPT6 1
Systematic Name YGR116W
Alias CRE2 2 , SSN20 3
Feature Type ORF, Verified
Description Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region; required for H3K36 trimethylation but not dimethylation by Set2p (4, 5, 6, 7, 8)
Name Description SuPpressor of Ty's 1
Chromosomal Location
ChrVII:720409 to 724764 | ORF Map | GBrowse
Gbrowse
Genetic position: 87.64 cM
Gene Ontology Annotations All SPT6 GO evidence and references
  View Computational GO annotations for SPT6
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Targets 2134 genes
Regulators 57 genes
Resources
Classical genetics
conditional
null
unspecified
Large-scale survey
null
overexpression
reduction of function
Resources
111 total interaction(s) for 69 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 36
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 6
  • Biochemical Activity: 1
  • Co-crystal Structure: 1
  • Co-fractionation: 1
  • Co-localization: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 3
  • Phenotypic Suppression: 3
  • Positive Genetic: 11
  • Synthetic Growth Defect: 15
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 10
  • Synthetic Rescue: 10

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,451
Molecular Weight (Da) 168,290
Isoelectric Point (pI) 4.73
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:720409 to 724764 | ORF Map | GBrowse
SGD ORF map
Genetic position: 87.64 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..4356 720409..724764 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003348
References cited on this page View Complete Literature Guide for SPT6
1) Winston F, et al.  (1984) Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae. Genetics 107(2):179-97
2) Denis CL  (1984) Identification of new genes involved in the regulation of yeast alcohol dehydrogenase II. Genetics 108(4):833-44
3) Neigeborn L, et al.  (1986) Suppressors of SNF2 mutations restore invertase derepression and cause temperature-sensitive lethality in yeast. Genetics 112(4):741-53
4) Bortvin A and Winston F  (1996) Evidence that Spt6p controls chromatin structure by a direct interaction with histones. Science 272(5267):1473-6
5) Hartzog GA, et al.  (1998) Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae. Genes Dev 12(3):357-69
6) Kaplan CD, et al.  (2003) Transcription elongation factors repress transcription initiation from cryptic sites. Science 301(5636):1096-9
7) Youdell ML, et al.  (2008) Roles for Ctk1 and Spt6 in regulating the different methylation states of histone H3 lysine 36. Mol Cell Biol 28(16):4915-26
8) Close D, et al.  (2011) Crystal Structures of the S. cerevisiae Spt6 Core and C-Terminal Tandem SH2 Domain. J Mol Biol 408(4):697-713