DAM1/YGR113W Summary Help

Standard Name DAM1 1
Systematic Name YGR113W
Feature Type ORF, Verified
Description Essential subunit of the Dam1 complex (aka DASH complex); cooperates with Duo1p to connect the DASH complex with the microtubules (MT); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments (2, 3, 4, 5, 6, 7, 8)
Name Description Duo1 And Mps1 interacting 1
Chromosomal Location
ChrVII:718893 to 719924 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All DAM1 GO evidence and references
  View Computational GO annotations for DAM1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
conditional
dominant negative
unspecified
Large-scale survey
conditional
null
overexpression
reduction of function
Resources
196 total interaction(s) for 102 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 13
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 15
  • Biochemical Activity: 12
  • Co-crystal Structure: 2
  • Co-fractionation: 2
  • Co-localization: 2
  • Co-purification: 7
  • PCA: 1
  • Reconstituted Complex: 15
  • Two-hybrid: 37

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 3
  • Negative Genetic: 43
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 3
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 25
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 343
Molecular Weight (Da) 38,422
Isoelectric Point (pI) 9.97
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:718893 to 719924 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2001-05-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1032 718893..719924 2011-02-03 2001-05-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003345
References cited on this page View Complete Literature Guide for DAM1
1) Hofmann C, et al.  (1998) Saccharomyces cerevisiae Duo1p and Dam1p, novel proteins involved in mitotic spindle function. J Cell Biol 143(4):1029-40
2) Li Y, et al.  (2002) The mitotic spindle is required for loading of the DASH complex onto the kinetochore. Genes Dev 16(2):183-97
3) Cheeseman IM, et al.  (2002) Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p. Cell 111(2):163-72
4) Courtwright AM and He X  (2002) Dam1 is the right one: phosphoregulation of kinetochore biorientation. Dev Cell 3(5):610-1
5) Miranda JL, et al.  (2005) The yeast DASH complex forms closed rings on microtubules. Nat Struct Mol Biol 12(2):138-43
6) Westermann S, et al.  (2005) Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complex. Mol Cell 17(2):277-90
7) Westermann S, et al.  (2006) The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends. Nature 440(7083):565-9
8) Miranda JJ, et al.  (2007) Protein arms in the kinetochore-microtubule interface of the yeast DASH complex. Mol Biol Cell 18(7):2503-10