DBF2/YGR092W Summary Help

Standard Name DBF2 1
Systematic Name YGR092W
Feature Type ORF, Verified
Description Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis; DBF2 has a paralog, DBF20, that arose from the whole genome duplication (2, 3, 4, 5, 6, 7, 8)
Name Description DumbBell Former 1
Chromosomal Location
ChrVII:668189 to 669907 | ORF Map | GBrowse
Gbrowse
Genetic position: 28 cM
Gene Ontology Annotations All DBF2 GO evidence and references
  View Computational GO annotations for DBF2
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
Regulators 112 genes
Resources
Classical genetics
conditional
null
reduction of function
Large-scale survey
null
Resources
455 total interaction(s) for 325 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 43
  • Affinity Capture-Western: 7
  • Biochemical Activity: 93
  • Co-localization: 12
  • Co-purification: 1
  • PCA: 3
  • Reconstituted Complex: 8
  • Two-hybrid: 11

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 29
  • Negative Genetic: 148
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 2
  • Positive Genetic: 25
  • Synthetic Growth Defect: 34
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 26
  • Synthetic Rescue: 8

Resources
Expression Summary
histogram
Resources
Length (a.a.) 572
Molecular Weight (Da) 66,148
Isoelectric Point (pI) 9.27
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:668189 to 669907 | ORF Map | GBrowse
SGD ORF map
Genetic position: 28 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1719 668189..669907 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003324
References cited on this page View Complete Literature Guide for DBF2
1) Johnston LH and Thomas AP  (1982) The isolation of new DNA synthesis mutants in the yeast Saccharomyces cerevisiae. Mol Gen Genet 186(3):439-44
2) Toyn JH and Johnston LH  (1994) The Dbf2 and Dbf20 protein kinases of budding yeast are activated after the metaphase to anaphase cell cycle transition. EMBO J 13(5):1103-13
3) Frenz LM, et al.  (2000) The budding yeast Dbf2 protein kinase localises to the centrosome and moves to the bud neck in late mitosis. J Cell Sci 113 Pt 19():3399-408
4) Visintin R and Amon A  (2001) Regulation of the mitotic exit protein kinases Cdc15 and Dbf2. Mol Biol Cell 12(10):2961-74
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Trcek T, et al.  (2011) Single-molecule mRNA decay measurements reveal promoter- regulated mRNA stability in yeast. Cell 147(7):1484-97
7) Oh Y, et al.  (2012) Mitotic exit kinase Dbf2 directly phosphorylates chitin synthase Chs2 to regulate cytokinesis in budding yeast. Mol Biol Cell 23(13):2445-56
8) Meitinger F, et al.  (2013) Dual function of the NDR-kinase Dbf2 in the regulation of the F-BAR protein Hof1 during cytokinesis. Mol Biol Cell 24(9):1290-304