SPT4/YGR063C Summary Help

Standard Name SPT4 1, 2
Systematic Name YGR063C
Feature Type ORF, Verified
Description Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions (3, 4, 5, 6, 7, 8, 9, 10, 11)
Name Description SuPpressor of Ty's 1
Chromosomal Location
ChrVII:617824 to 617516 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 40 cM
Gene Ontology Annotations All SPT4 GO evidence and references
  View Computational GO annotations for SPT4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
reduction of function
Large-scale survey
385 total interaction(s) for 232 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 67
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 12
  • Biochemical Activity: 1
  • Co-crystal Structure: 1
  • PCA: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 64
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 120
  • Positive Genetic: 11
  • Synthetic Growth Defect: 33
  • Synthetic Lethality: 45
  • Synthetic Rescue: 15

Expression Summary
Length (a.a.) 102
Molecular Weight (Da) 11,158
Isoelectric Point (pI) 4.9
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:617824 to 617516 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 40 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..309 617824..617516 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003295
References cited on this page View Complete Literature Guide for SPT4
1) Winston F, et al.  (1984) Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae. Genetics 107(2):179-97
2) Fassler, J. and Winston, F.  (1989) Personal Communication, Mortimer Map Edition 10
3) Lindstrom DL, et al.  (2003) Dual roles for Spt5 in pre-mRNA processing and transcription elongation revealed by identification of Spt5-associated proteins. Mol Cell Biol 23(4):1368-78
4) Rondon AG, et al.  (2003) Molecular evidence for a positive role of Spt4 in transcription elongation. EMBO J 22(3):612-20
5) Crotti LB and Basrai MA  (2004) Functional roles for evolutionarily conserved Spt4p at centromeres and heterochromatin in Saccharomyces cerevisiae. EMBO J 23(8):1804-14
6) Schneider DA, et al.  (2006) RNA polymerase II elongation factors Spt4p and Spt5p play roles in transcription elongation by RNA polymerase I and rRNA processing. Proc Natl Acad Sci U S A 103(34):12707-12
7) Ding B, et al.  (2010) The C-terminal repeat domain of Spt5 plays an important role in suppression of Rad26-independent transcription coupled repair. J Biol Chem 285(8):5317-26
8) Shen Z, et al.  (2010) Cotranscriptional recruitment of She2p by RNA pol II elongation factor Spt4-Spt5/DSIF promotes mRNA localization to the yeast bud. Genes Dev 24(17):1914-26
9) Anderson SJ, et al.  (2011) The transcription elongation factor Spt5 influences transcription by RNA polymerase I positively and negatively. J Biol Chem 286(21):18816-24
10) Lepore N and Lafontaine DL  (2011) A Functional Interface at the rDNA Connects rRNA Synthesis, Pre-rRNA Processing and Nucleolar Surveillance in Budding Yeast. PLoS One 6(9):e24962
11) Liu CR, et al.  (2012) Spt4 is selectively required for transcription of extended trinucleotide repeats. Cell 148(4):690-701