SPT4/YGR063C Summary 
| Standard Name | SPT4 1, 2 |
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| Systematic Name | YGR063C |
| Feature Type | ORF, Verified |
| Description | Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions (3, 4, 5, 6, 7, 8, 9, 10, 11) |
| Name Description | SuPpressor of Ty's 1 |
| Chromosomal Location | |
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| Note: this feature is encoded on the Crick strand. | |
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| Genetic position: 40 cM |
Gene Ontology Annotations All SPT4 GO evidence and references
| View Computational GO annotations for SPT4 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated |
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| Cellular Component | |
| Manually curated |
Mutant phenotypes All SPT4 Phenotype evidence and references
interactions All SPT4 Interaction evidence and references
| 378 total interaction(s) for 228 unique genes/features. | |
| Physical Interactions |
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| Genetic Interactions |
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| Resources |
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Expression Summary
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| Resources |
Protein Information All SPT4 Protein evidence and references
| Localization | |
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| Phosphorylation | PhosphoGRID | PhosphoPep Database |
| Structure | |
| Homologs |
sequence information
| Note: this feature is encoded on the Crick strand. | |||||||||||||
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| Genetic position: 40 cM | |||||||||||||
| Last Update | Coordinates: 2011-02-03 | Sequence: 1996-07-31 | ||||||||||||
| Subfeature details |
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| Retrieve sequences | |||||||||||||
Analyze Sequence
| S288C only | |
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| S288C vs. other species | |
| S288C vs. other strains |
Resources
| External Links | All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB |
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| Primary SGDID | S000003295 |
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References cited on this page View Complete Literature Guide for SPT4
| 1) | Winston F, et al. (1984) Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae. Genetics 107(2):179-97 |
| 2) | Fassler, J. and Winston, F. (1989) Personal Communication, Mortimer Map Edition 10 |
| 3) | Lindstrom DL, et al. (2003) Dual roles for Spt5 in pre-mRNA processing and transcription elongation revealed by identification of Spt5-associated proteins. Mol Cell Biol 23(4):1368-78 |
| 4) | Rondon AG, et al. (2003) Molecular evidence for a positive role of Spt4 in transcription elongation. EMBO J 22(3):612-20 |
| 5) | Crotti LB and Basrai MA (2004) Functional roles for evolutionarily conserved Spt4p at centromeres and heterochromatin in Saccharomyces cerevisiae. EMBO J 23(8):1804-14 |
| 6) | Schneider DA, et al. (2006) RNA polymerase II elongation factors Spt4p and Spt5p play roles in transcription elongation by RNA polymerase I and rRNA processing. Proc Natl Acad Sci U S A 103(34):12707-12 |
| 7) | Ding B, et al. (2010) The C-terminal repeat domain of Spt5 plays an important role in suppression of Rad26-independent transcription coupled repair. J Biol Chem 285(8):5317-26 |
| 8) | Shen Z, et al. (2010) Cotranscriptional recruitment of She2p by RNA pol II elongation factor Spt4-Spt5/DSIF promotes mRNA localization to the yeast bud. Genes Dev 24(17):1914-26 |
| 9) | Anderson SJ, et al. (2011) The transcription elongation factor Spt5 influences transcription by RNA polymerase I positively and negatively. J Biol Chem 286(21):18816-24 |
| 10) | Lepore N and Lafontaine DL (2011) A Functional Interface at the rDNA Connects rRNA Synthesis, Pre-rRNA Processing and Nucleolar Surveillance in Budding Yeast. PLoS One 6(9):e24962 |
| 11) | Liu CR, et al. (2012) Spt4 is selectively required for transcription of extended trinucleotide repeats. Cell 148(4):690-701 |
