PRP18/YGR006W Summary Help

Standard Name PRP18 1
Systematic Name YGR006W
Feature Type ORF, Verified
Description Splicing factor and component of snRNP U5; factor involved in the positioning of the 3' splice site during the second catalytic step of splicing; interacts with Slu7p (2, 3, 4, 5)
Name Description Pre-mRNA Processing 1
Chromosomal Location
ChrVII:506070 to 506825 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PRP18 GO evidence and references
  View Computational GO annotations for PRP18
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 57 genes
Resources
Classical genetics
conditional
Large-scale survey
null
Resources
61 total interaction(s) for 44 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Co-fractionation: 1
  • Co-purification: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 23
  • Phenotypic Enhancement: 3
  • Positive Genetic: 3
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 12
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 251
Molecular Weight (Da) 28,377
Isoelectric Point (pI) 6.64
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:506070 to 506825 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2004-01-22
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..756 506070..506825 2011-02-03 2004-01-22
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003238
References cited on this page View Complete Literature Guide for PRP18
1) Vijayraghavan U, et al.  (1989) Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev 3(8):1206-16
2) Horowitz DS and Abelson J  (1993) A U5 small nuclear ribonucleoprotein particle protein involved only in the second step of pre-mRNA splicing in Saccharomyces cerevisiae. Mol Cell Biol 13(5):2959-70
3) Umen JG and Guthrie C  (1995) Prp16p, Slu7p, and Prp8p interact with the 3' splice site in two distinct stages during the second catalytic step of pre-mRNA splicing. RNA 1(6):584-97
4) James SA, et al.  (2002) How Slu7 and Prp18 cooperate in the second step of yeast pre-mRNA splicing. RNA 8(8):1068-77
5) Umen JG and Guthrie C  (1995) The second catalytic step of pre-mRNA splicing. RNA 1(9):869-85