Standard Name	PRP18 1
Systematic Name	YGR006W
Feature Type	ORF, Verified
Description	Splicing factor involved in the positioning of the 3' splice site during the second catalytic step of splicing, part of snRNP U5, interacts with Slu7p (2, 3, 4, 5)
Name Description	Pre-mRNA Processing 1

Chromosomal Location	ChrVII:506070 to 506825 | ORF Map | GBrowse

	View Computational GO annotations for PRP18
Molecular Function
Manually curated	contributes_to second spliceosomal transesterification activity (IDA, IGI)
Biological Process
Manually curated	generation of catalytic spliceosome for second transesterification step (IDA) mRNA splicing, via spliceosome (IMP) nuclear retention of unspliced pre-mRNA at the site of transcription (IGI, IMP)
Cellular Component
Manually curated	U4/U6 x U5 tri-snRNP complex (IDA) U5 snRNP (IDA)

Classical genetics
conditional	splicing intermediate accumulation: abnormal heat sensitivity: increased
Large-scale survey
null	bud morphology: abnormal cell size: increased competitive fitness: decreased haploinsufficient heat sensitivity: increased Fus-Mid-GFP distribution: abnormal resistance to St. John's Wort extract: decreased resistance to L-1,4-dithiothreitol: decreased resistance to pyrrolidine dithiocarbamate: decreased utilization of nitrogen source: absent vacuolar morphology: abnormal vegetative growth: decreased rate viable
Resources	PROPHECY | PhenoM | SCMD | ScreenTroll | Yeast Fitness Database

	61 total interaction(s) for 44 unique genes/features.
Physical Interactions	Affinity Capture-MS: 4 Affinity Capture-RNA: 1 Affinity Capture-Western: 1 Co-fractionation: 1 Co-purification: 1 Reconstituted Complex: 1 Two-hybrid: 6
Genetic Interactions	Dosage Rescue: 2 Negative Genetic: 23 Phenotypic Enhancement: 3 Positive Genetic: 3 Synthetic Growth Defect: 1 Synthetic Lethality: 12 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrVII:506070 to 506825 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 2004-01-22
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..756 506070..506825 2011-02-03 2004-01-22
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000003238