PRP18/YGR006W Summary Help

Standard Name PRP18 1
Systematic Name YGR006W
Feature Type ORF, Verified
Description Splicing factor and component of snRNP U5; factor involved in the positioning of the 3' splice site during the second catalytic step of splicing; interacts with Slu7p (2, 3, 4, 5)
Name Description Pre-mRNA Processing 1
Chromosomal Location
ChrVII:506070 to 506825 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PRP18 GO evidence and references
  View Computational GO annotations for PRP18
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
conditional
Large-scale survey
null
Resources
62 total interaction(s) for 44 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Co-fractionation: 1
  • Co-purification: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 23
  • Phenotypic Enhancement: 4
  • Positive Genetic: 3
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 12
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 251
Molecular Weight (Da) 28,377
Isoelectric Point (pI) 6.64
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:506070 to 506825 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2004-01-22
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..756 506070..506825 2011-02-03 2004-01-22
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003238
References cited on this page View Complete Literature Guide for PRP18
1) Vijayraghavan U, et al.  (1989) Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev 3(8):1206-16
2) Horowitz DS and Abelson J  (1993) A U5 small nuclear ribonucleoprotein particle protein involved only in the second step of pre-mRNA splicing in Saccharomyces cerevisiae. Mol Cell Biol 13(5):2959-70
3) Umen JG and Guthrie C  (1995) Prp16p, Slu7p, and Prp8p interact with the 3' splice site in two distinct stages during the second catalytic step of pre-mRNA splicing. RNA 1(6):584-97
4) James SA, et al.  (2002) How Slu7 and Prp18 cooperate in the second step of yeast pre-mRNA splicing. RNA 8(8):1068-77
5) Umen JG and Guthrie C  (1995) The second catalytic step of pre-mRNA splicing. RNA 1(9):869-85