HFM1/YGL251C Summary

HFM1/YGL251C Summary

Standard Name	HFM1
Systematic Name	YGL251C
Alias	MER3 1
Feature Type	ORF, Verified
Description	Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity (2, 3, 4, 5)
Name Description	Helicase Family Member

Chromosomal Location	ChrVII:31636 to 27921 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All HFM1 GO evidence and references

	View Computational GO annotations for HFM1
Molecular Function
Manually curated	DNA helicase activity (IDA)
Biological Process
Manually curated	DNA unwinding involved in replication (IDA) meiosis (IDA) reciprocal meiotic recombination (IMP) synapsis (IGI, IMP)
Cellular Component
Manually curated	nucleus (IDA)

Mutant phenotypes All HFM1 Phenotype evidence and references

Classical genetics
null	chronological lifespan: increased meiotic recombination: decreased Zip1p distribution: abnormal Ptr2p-GFP distribution: abnormal spore germination: decreased
Large-scale survey
null	competitive fitness: normal competitive fitness: decreased nutrient utilization: decreased resistance to trichothecene: increased resistance to dimethyl sulfoxide: decreased respiratory growth: absent toxin resistance: increased viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All HFM1 Interaction evidence and references

	14 total interaction(s) for 14 unique genes/features.
Physical Interactions	Affinity Capture-MS: 3 Affinity Capture-RNA: 1 PCA: 1 Two-hybrid: 2
Genetic Interactions	Dosage Rescue: 2 Negative Genetic: 3 Phenotypic Suppression: 1 Positive Genetic: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All HFM1 Protein evidence and references

Localization	YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrVII:31636 to 27921 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2000-07-14 | Sequence: 2000-07-14

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..58	31636..31579	2000-07-14	2000-07-14
Intron	59..210	31578..31427	2000-07-14	2000-07-14
CDS	211..3716	31426..27921	2000-07-14	2000-07-14

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003220

References cited on this page View Complete Literature Guide for HFM1

1)	Nakagawa T and Ogawa H (1999) The Saccharomyces cerevisiae MER3 gene, encoding a novel helicase-like protein, is required for crossover control in meiosis. EMBO J 18(20):5714-23
2)	Coissac E, et al. (1996) Sequence of a 39,411 bp DNA fragment covering the left end of chromosome VII of Saccharomyces cerevisiae. Yeast 12(15):1555-62
3)	Nakagawa T, et al. (2001) The MER3 helicase involved in meiotic crossing over is stimulated by single-stranded DNA-binding proteins and unwinds DNA in the 3' to 5' direction. J Biol Chem 276(34):31487-93
4)	Nakagawa T and Kolodner RD (2002) Saccharomyces cerevisiae Mer3 is a DNA helicase involved in meiotic crossing over. Mol Cell Biol 22(10):3281-91
5)	Nakagawa T and Kolodner RD (2002) The MER3 DNA helicase catalyzes the unwinding of holliday junctions. J Biol Chem 277(31):28019-24