DOC1/YGL240W Summary Help

Standard Name DOC1 1
Systematic Name YGL240W
Alias APC10
Feature Type ORF, Verified
Description Processivity factor; required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain (1, 2, 3, 4, 5)
Name Description Destruction Of Cyclin B 1
Chromosomal Location
ChrVII:48613 to 49365 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All DOC1 GO evidence and references
  View Computational GO annotations for DOC1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Classical genetics
conditional
null
Large-scale survey
null
Resources
130 total interaction(s) for 65 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 43
  • Affinity Capture-Western: 9
  • Biochemical Activity: 3
  • Co-crystal Structure: 2
  • Co-fractionation: 1
  • PCA: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 12

Genetic Interactions
  • Dosage Lethality: 6
  • Dosage Rescue: 3
  • Negative Genetic: 5
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 3
  • Synthetic Growth Defect: 10
  • Synthetic Lethality: 24
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 250
Molecular Weight (Da) 28,776
Isoelectric Point (pI) 5.54
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:48613 to 49365 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2003-09-22 | Sequence: 2003-09-22
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..753 48613..49365 2003-09-22 2003-09-22
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003209
References cited on this page View Complete Literature Guide for DOC1
1) Hwang LH and Murray AW  (1997) A novel yeast screen for mitotic arrest mutants identifies DOC1, a new gene involved in cyclin proteolysis. Mol Biol Cell 8(10):1877-87
2) Grossberger R, et al.  (1999) Characterization of the DOC1/APC10 subunit of the yeast and the human anaphase-promoting complex. J Biol Chem 274(20):14500-7
3) Au SW, et al.  (2002) Implications for the ubiquitination reaction of the anaphase-promoting complex from the crystal structure of the Doc1/Apc10 subunit. J Mol Biol 316(4):955-68
4) Carroll CW and Morgan DO  (2002) The Doc1 subunit is a processivity factor for the anaphase-promoting complex. Nat Cell Biol 4(11):880-7
5) Passmore LA, et al.  (2003) Doc1 mediates the activity of the anaphase-promoting complex by contributing to substrate recognition. EMBO J 22(4):786-96