DOC1/YGL240W Summary

DOC1/YGL240W Summary

Standard Name	DOC1
Systematic Name	YGL240W
Alias	APC10
Feature Type	ORF, Verified
Description	Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain (1, 2, 3, 4, 5)
Name Description	Destruction Of Cyclin B 1

Chromosomal Location	ChrVII:48613 to 49365 \| ORF Map \| GBrowse

Gene Ontology Annotations All DOC1 GO evidence and references

	View Computational GO annotations for DOC1
Molecular Function
Manually curated	enzyme regulator activity (IDA) contributes_to mitotic anaphase-promoting complex activity (IDA)
Biological Process
Manually curated	activation of mitotic anaphase-promoting complex activity (IDA, IMP) anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process (IDA, IMP) chromatin assembly (IGI) cyclin catabolic process (IMP)
Cellular Component
Manually curated	anaphase-promoting complex (IDA)
High-throughput	mitochondrion (IDA)

Mutant phenotypes All DOC1 Phenotype evidence and references

Classical genetics
conditional	cell cycle progression through the G2/M phase transition: arrested Clb2p accumulation: increased
null	cell cycle passage through the metaphase-anaphase transition: delayed chromosome/plasmid maintenance: abnormal colony sectoring: increased mitochondrial genome maintenance: abnormal vegetative growth: decreased rate
Large-scale survey
null	acid pH resistance: decreased proton accumulation: decreased proton accumulation: increased glycogen accumulation: decreased chitin deposition: increased competitive fitness: decreased haploinsufficient heat sensitivity: increased resistance to Calcofluor White: decreased resistance to hydroxyurea: decreased resistance to ethanol: decreased resistance to arsenite(3-): decreased resistance to 4-chlorophenol: decreased resistance to 2,4-dichlorophenol: decreased resistance to cisplatin: decreased resistance to L-1,4-dithiothreitol: decreased resistance to doxorubicin: decreased respiratory growth: decreased rate respiratory growth: absent toxin resistance: decreased utilization of carbon source: decreased vegetative growth: decreased rate viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All DOC1 Interaction evidence and references

	129 total interaction(s) for 64 unique genes/features.
Physical Interactions	Affinity Capture-MS: 43 Affinity Capture-Western: 9 Biochemical Activity: 3 Co-crystal Structure: 2 Co-fractionation: 1 PCA: 1 Reconstituted Complex: 3 Two-hybrid: 12
Genetic Interactions	Dosage Lethality: 6 Dosage Rescue: 3 Negative Genetic: 5 Phenotypic Enhancement: 2 Phenotypic Suppression: 2 Synthetic Growth Defect: 10 Synthetic Lethality: 24 Synthetic Rescue: 3
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All DOC1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrVII:48613 to 49365 | |

SGD ORF map

Last Update

Coordinates: 2003-09-22 | Sequence: 2003-09-22

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..753	48613..49365	2003-09-22	2003-09-22

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003209

References cited on this page View Complete Literature Guide for DOC1

1)	Hwang LH and Murray AW (1997) A novel yeast screen for mitotic arrest mutants identifies DOC1, a new gene involved in cyclin proteolysis. Mol Biol Cell 8(10):1877-87
2)	Grossberger R, et al. (1999) Characterization of the DOC1/APC10 subunit of the yeast and the human anaphase-promoting complex. J Biol Chem 274(20):14500-7
3)	Au SW, et al. (2002) Implications for the ubiquitination reaction of the anaphase-promoting complex from the crystal structure of the Doc1/Apc10 subunit. J Mol Biol 316(4):955-68
4)	Carroll CW and Morgan DO (2002) The Doc1 subunit is a processivity factor for the anaphase-promoting complex. Nat Cell Biol 4(11):880-7
5)	Passmore LA, et al. (2003) Doc1 mediates the activity of the anaphase-promoting complex by contributing to substrate recognition. EMBO J 22(4):786-96