SEC15/YGL233W Summary Help

Standard Name SEC15 1
Systematic Name YGL233W
Feature Type ORF, Verified
Description Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase (2, 3, 4, 5)
Name Description SECretory 6
Chromosomal Location
ChrVII:59122 to 61854 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SEC15 GO evidence and references
  View Computational GO annotations for SEC15
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
conditional
reduction of function
repressible
Large-scale survey
null
overexpression
reduction of function
repressible
Resources
253 total interaction(s) for 133 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 22
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 29
  • Co-fractionation: 6
  • Co-localization: 1
  • Co-purification: 1
  • PCA: 1
  • Reconstituted Complex: 7
  • Two-hybrid: 12

Genetic Interactions
  • Dosage Growth Defect: 5
  • Dosage Lethality: 6
  • Dosage Rescue: 23
  • Negative Genetic: 32
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 1
  • Positive Genetic: 3
  • Synthetic Growth Defect: 23
  • Synthetic Lethality: 73
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 910
Molecular Weight (Da) 105,063
Isoelectric Point (pI) 4.63
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:59122 to 61854 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2004-07-15 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2733 59122..61854 2004-07-15 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003202
References cited on this page View Complete Literature Guide for SEC15
1) Novick P, et al.  (1980) Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell 21(1):205-15
2) Bowser R, et al.  (1992) Sec8p and Sec15p are components of a plasma membrane-associated 19.5S particle that may function downstream of Sec4p to control exocytosis. J Cell Biol 118(5):1041-56
3) TerBush DR, et al.  (1996) The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae. EMBO J 15(23):6483-94
4) TerBush DR and Novick P  (1995) Sec6, Sec8, and Sec15 are components of a multisubunit complex which localizes to small bud tips in Saccharomyces cerevisiae. J Cell Biol 130(2):299-312
5) Guo W, et al.  (1999) The exocyst is an effector for Sec4p, targeting secretory vesicles to sites of exocytosis. EMBO J 18(4):1071-80
6) Novick P and Schekman R  (1979) Secretion and cell-surface growth are blocked in a temperature-sensitive mutant of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 76(4):1858-62