YPT32/YGL210W Summary Help

Standard Name YPT32 1
Systematic Name YGL210W
Feature Type ORF, Verified
Description Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6 and see Summary Paragraph)
Name Description Yeast Protein Two 1
Chromosomal Location
ChrVII:93792 to 94460 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All YPT32 GO evidence and references
  View Computational GO annotations for YPT32
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
106 total interaction(s) for 63 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 6
  • Affinity Capture-Western: 5
  • Biochemical Activity: 1
  • Co-purification: 2
  • PCA: 2
  • Protein-RNA: 1
  • Reconstituted Complex: 11
  • Two-hybrid: 12

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Lethality: 2
  • Dosage Rescue: 14
  • Negative Genetic: 25
  • Phenotypic Enhancement: 2
  • Positive Genetic: 3
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 3
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 222
Molecular Weight (Da) 24,520
Isoelectric Point (pI) 5.22
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:93792 to 94460 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..669 93792..94460 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003178
SUMMARY PARAGRAPH for YPT32

Rab proteins are small Ras-related GTPases that function in multiple stages in membrane traffic. Rabs continuously cycle between the cytosol and membranes. The GDP-bound form of the Rab is complexed with guanine nucleotide dissociation inhibitor(GDI) in the cytosol and are recruited to the membranes with the help of GDF (GDI Displacement Factor) and by their prenylation by geranylgeranyl transferase II (GGTase II). The membrane-bound Rab is activated to the GTP-bound form by the guanine nucleotide exchange factors (GEFs) which then goes on to interact with downstream effectors. The GTPase-activating protein (GAP) stimulates the hydrolysis of GTP to GDP to inactivate the Rab. Eleven Rabs have been identified in yeast and more than 60 in mammalian cells (reviewed in 7, 8, 9).

Last updated: 2010-05-18 Contact SGD

References cited on this page View Complete Literature Guide for YPT32
1) Benli M, et al.  (1996) Two GTPase isoforms, Ypt31p and Ypt32p, are essential for Golgi function in yeast. EMBO J 15(23):6460-75
2) Jedd G, et al.  (1997) Two new Ypt GTPases are required for exit from the yeast trans-Golgi compartment. J Cell Biol 137(3):563-80
3) Lazar T, et al.  (1997) Vesicular transport: how many Ypt/Rab-GTPases make a eukaryotic cell? Trends Biochem Sci 22(12):468-72
4) Wang W and Ferro-Novick S  (2002) A Ypt32p exchange factor is a putative effector of Ypt1p. Mol Biol Cell 13(9):3336-43
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7) Cai H, et al.  (2007) Coats, tethers, Rabs, and SNAREs work together to mediate the intracellular destination of a transport vesicle. Dev Cell 12(5):671-82
8) Grosshans BL, et al.  (2006) Rabs and their effectors: achieving specificity in membrane traffic. Proc Natl Acad Sci U S A 103(32):11821-7
9) Bialek-Wyrzykowska U, et al.  (2000) Low levels of Ypt protein prenylation cause vesicle polarization defects and thermosensitive growth that can be suppressed by genes involved in cell wall maintenance. Mol Microbiol 35(6):1295-311