GCN1/YGL195W Summary Help

Standard Name GCN1 1
Systematic Name YGL195W
Alias NDR1 2 , AAS103 2
Feature Type ORF, Verified
Description Positive regulator of the Gcn2p kinase activity; forms a complex with Gcn20p; proposed to stimulate Gcn2p activation by an uncharged tRNA (3, 4)
Name Description General Control Nonderepressible 5
Chromosomal Location
ChrVII:131525 to 139543 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All GCN1 GO evidence and references
  View Computational GO annotations for GCN1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
null
overexpression
reduction of function
Large-scale survey
null
Resources
403 total interaction(s) for 286 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 96
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 8
  • Co-fractionation: 3
  • Co-purification: 1
  • PCA: 5
  • Reconstituted Complex: 3
  • Two-hybrid: 9

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 210
  • Positive Genetic: 51
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 2,672
Molecular Weight (Da) 296,695
Isoelectric Point (pI) 5.02
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:131525 to 139543 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..8019 131525..139543 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003163
References cited on this page View Complete Literature Guide for GCN1
1) Skvirsky RC, et al.  (1986) A new negative control gene for amino acid biosynthesis in Saccharomyces cerevisiae. Curr Genet 10(7):495-501
2) Thireos G, et al.  (1984) 5' untranslated sequences are required for the translational control of a yeast regulatory gene. Proc Natl Acad Sci U S A 81(16):5096-100
3) Kubota H, et al.  (2001) Budding yeast GCN1 binds the GI domain to activate the eIF2alpha kinase GCN2. J Biol Chem 276(20):17591-6
4) Garcia-Barrio M, et al.  (2000) Association of GCN1-GCN20 regulatory complex with the N-terminus of eIF2alpha kinase GCN2 is required for GCN2 activation. EMBO J 19(8):1887-99
5) Lucchini G, et al.  (1984) Positive regulatory interactions of the HIS4 gene of Saccharomyces cerevisiae. Mol Cell Biol 4(7):1326-33