GTS1/YGL181W Summary

GTS1/YGL181W Summary

Standard Name	GTS1 1 (see Nomenclature conflict Note)
Systematic Name	YGL181W
Alias	FHT1 2 , LSR1 3
Feature Type	ORF, Verified
Description	Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations (1, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
Name Description	Glycine Threonine Serine repeat protein 1

Chromosomal Location	ChrVII:157906 to 159096 \| ORF Map \| GBrowse

Gene Ontology Annotations All GTS1 GO evidence and references

	View Computational GO annotations for GTS1
Molecular Function
Manually curated	ARF GTPase activator activity (IMP, ISS) repressing transcription factor binding (IPI) transcription factor binding transcription factor activity (IMP, IPI)
Biological Process
Manually curated	endocytosis (IMP) negative regulation of phosphatidylinositol biosynthetic process (IMP) positive regulation of transcription from RNA polymerase II promoter (IMP) regulation of transcription from RNA polymerase II promoter (IPI) ultradian rhythm (IMP)
Cellular Component
Manually curated	actin cortical patch (IDA) cytoplasm (IPI) nucleus (IDA)

Regulatory Role

Regulatory modules	predicted: stressResponse (607)

Resources	ScerTF \| YeTFaSCo

Mutant phenotypes All GTS1 Phenotype evidence and references

Classical genetics
null	dessication resistance: decreased endocytosis: decreased vacuolar morphology: abnormal viable
Large-scale survey
null	auxotrophy competitive fitness: decreased resistance to cycloheximide: decreased starvation resistance: decreased toxin resistance: increased vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All GTS1 Interaction evidence and references

	76 total interaction(s) for 60 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Affinity Capture-RNA: 2 Affinity Capture-Western: 7 Biochemical Activity: 2 Protein-RNA: 2 Reconstituted Complex: 1 Two-hybrid: 29
Genetic Interactions	Dosage Growth Defect: 2 Dosage Rescue: 4 Negative Genetic: 14 Phenotypic Enhancement: 1 Phenotypic Suppression: 1 Positive Genetic: 1 Synthetic Growth Defect: 2 Synthetic Lethality: 6 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All GTS1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrVII:157906 to 159096 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1191	157906..159096	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003149

NOMENCLATURE CONFLICT NOTE

Name	Relevance	Description
LSR1	Nomenclature conflict	The LSR1 name has been used both for the U2 snRNA encoded by LSR1/snR20 and for GTS1/YGL181W.

References cited on this page View Complete Literature Guide for GTS1

1)	Mitsui K, et al. (1994) The GTS1 gene, which contains a Gly-Thr repeat, affects the timing of budding and cell size of the yeast Saccharomyces cerevisiae. Mol Cell Biol 14(8):5569-78
2)	Iha H, et al. (2001) Functional correlation between the nuclear localization of Fht1p and its flocculation and heat tolerance activities in budding yeast Saccharomyces cerevisiae. J Biomed Sci 8(5):416-20
3)	SGD (2009) Gene name standardized from a GenBank/EMBL/DDBJ record
4)	Yaguchi S, et al. (1996) The pleiotropic effect of the GTS1 gene product on heat tolerance, sporulation and the life span of Saccharomyces cerevisiae. Biochem Biophys Res Commun 218(1):234-7
5)	Bossier P, et al. (1997) Constitutive flocculation in Saccharomyces cerevisiae through overexpression of the GTS1 gene, coding for a 'Glo'-type Zn-finger-containing protein. Yeast 13(8):717-25
6)	Tonozuka H, et al. (2001) Analysis of the upstream regulatory region of the GTS1 gene required for its oscillatory expression. J Biochem 130(5):589-95
7)	Saito T, et al. (2002) Regulation of the Gts1p level by the ubiquitination system to maintain metabolic oscillations in the continuous culture of yeast. J Biol Chem 277(37):33624-31
8)	Adams CA, et al. (2003) The Gts1 protein stabilizes the autonomous oscillator in yeast. Yeast 20(6):463-70
9)	Shen H, et al. (2006) The mechanism by which overexpression of Gts1p induces flocculation in a FLO8-inactive strain of the yeast Saccharomyces cerevisiae. FEMS Yeast Res 6(6):914-23
10)	Xu Z and Tsurugi K (2007) Role of Gts1p in regulation of energy-metabolism oscillation in continuous cultures of the yeast Saccharomyces cerevisiae. Yeast 24(3):161-70
11)	Yaguchi S, et al. (2007) Localization of Gts1p in cortical actin patches of yeast and its possible role in endocytosis. Eur J Cell Biol 86(5):275-85
12)	Smaczynska-de Rooij II, et al. (2008) Yeast Arf3p Modulates Plasma Membrane PtdIns(4,5)P2 Levels to Facilitate Endocytosis. Traffic 9(4):559-73
13)	Toret CP, et al. (2008) Multiple pathways regulate endocytic coat disassembly in Saccharomyces cerevisiae for optimal downstream trafficking. Traffic 9(5):848-59