SAE2/YGL175C Summary Help

Standard Name SAE2 1
Systematic Name YGL175C
Alias COM1 2
Feature Type ORF, Verified
Description Endonuclease required for telomere elongation; also required for telomeric 5' C-rich strand resection; involved in processing hairpin DNA structures with MRX complex; involved in double-strand break repair; required for normal resistance to DNA-damaging agents; exists in form of inactive oligomers that are transiently released into smaller active units by a series of phosphorylations; DNA damage triggers removal of Sae2p ensuring that active Sae2p is present only transiently (1, 2, 3, 4, 5, 6, 7)
Name Description Sporulation in the Absence of spo Eleven 1
Chromosomal Location
ChrVII:174322 to 173285 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All SAE2 GO evidence and references
  View Computational GO annotations for SAE2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Regulators 6 genes
Classical genetics
reduction of function
Large-scale survey
148 total interaction(s) for 79 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 3
  • Far Western: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 8

Genetic Interactions
  • Dosage Growth Defect: 6
  • Dosage Lethality: 3
  • Dosage Rescue: 4
  • Negative Genetic: 46
  • Phenotypic Enhancement: 17
  • Phenotypic Suppression: 11
  • Positive Genetic: 1
  • Synthetic Growth Defect: 20
  • Synthetic Lethality: 18
  • Synthetic Rescue: 4

Expression Summary
Length (a.a.) 345
Molecular Weight (Da) 40,097
Isoelectric Point (pI) 6.54
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:174322 to 173285 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1038 174322..173285 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003143
References cited on this page View Complete Literature Guide for SAE2
1) McKee AH and Kleckner N  (1997) A general method for identifying recessive diploid-specific mutations in Saccharomyces cerevisiae, its application to the isolation of mutants blocked at intermediate stages of meiotic prophase and characterization of a new gene SAE2. Genetics 146(3):797-816
2) Prinz S, et al.  (1997) Isolation of COM1, a new gene required to complete meiotic double-strand break-induced recombination in Saccharomyces cerevisiae. Genetics 146(3):781-95
3) Rattray AJ, et al.  (2001) Fidelity of mitotic double-strand-break repair in Saccharomyces cerevisiae: a role for SAE2/COM1. Genetics 158(1):109-22
4) Baroni E, et al.  (2004) The functions of budding yeast Sae2 in the DNA damage response require Mec1- and Tel1-dependent phosphorylation. Mol Cell Biol 24(10):4151-65
5) Lengsfeld BM, et al.  (2007) Sae2 Is an Endonuclease that Processes Hairpin DNA Cooperatively with the Mre11/Rad50/Xrs2 Complex. Mol Cell 28(4):638-51
6) Bonetti D, et al.  (2009) Multiple pathways regulate 3' overhang generation at S. cerevisiae telomeres. Mol Cell 35(1):70-81
7) Fu Q, et al.  (2014) Phosphorylation-regulated transitions in an oligomeric state control the activity of the sae2 DNA repair enzyme. Mol Cell Biol 34(5):778-93