XRN1/YGL173C Summary Help

Standard Name XRN1 1
Systematic Name YGL173C
Alias DST2 , RAR5 , SEP1 , SKI1 2 , KEM1 3
Feature Type ORF, Verified
Description Evolutionarily-conserved 5'-3' exonuclease; component of cytoplasmic processing (P) bodies involved in mRNA decay; also enters the nucleus and positively regulates transcription initiation and elongation; plays a role in microtubule-mediated processes, filamentous growth, ribosomal RNA maturation, and telomere maintenance; activated by the scavenger decapping enzyme Dcs1p (1, 4, 5, 6, 7, 8, 9, 10)
Name Description eXoRiboNuclease 1
Chromosomal Location
ChrVII:180113 to 175527 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: -111 cM
Gene Ontology Annotations All XRN1 GO evidence and references
  View Computational GO annotations for XRN1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
reduction of function
Large-scale survey
707 total interaction(s) for 456 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 143
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 2
  • Co-purification: 2
  • PCA: 3
  • Reconstituted Complex: 3
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 3
  • Negative Genetic: 336
  • Phenotypic Enhancement: 15
  • Phenotypic Suppression: 24
  • Positive Genetic: 79
  • Synthetic Growth Defect: 43
  • Synthetic Lethality: 33
  • Synthetic Rescue: 7

Expression Summary
Length (a.a.) 1,528
Molecular Weight (Da) 175,458
Isoelectric Point (pI) 7.5
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:180113 to 175527 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: -111 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..4587 180113..175527 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003141
References cited on this page View Complete Literature Guide for XRN1
1) Larimer FW and Stevens A  (1990) Disruption of the gene XRN1, coding for a 5'----3' exoribonuclease, restricts yeast cell growth. Gene 95(1):85-90
2) Toh-E A, et al.  (1978) Chromosomal superkiller mutants of Saccharomyces cerevisiae. J Bacteriol 136(3):1002-7
3) Kim J, et al.  (1990) kem mutations affect nuclear fusion in Saccharomyces cerevisiae. Genetics 126(4):799-812
4) Solinger JA, et al.  (1999) Active-site mutations in the Xrn1p exoribonuclease of Saccharomyces cerevisiae reveal a specific role in meiosis. Mol Cell Biol 19(9):5930-42
5) Geerlings TH, et al.  (2000) The final step in the formation of 25S rRNA in Saccharomyces cerevisiae is performed by 5'-->3' exonucleases. RNA 6(12):1698-703
6) Kim J and Kim J  (2002) KEM1 is involved in filamentous growth of Saccharomyces cerevisiae. FEMS Microbiol Lett 216(1):33-8
7) Sheth U and Parker R  (2003) Decapping and decay of messenger RNA occur in cytoplasmic processing bodies. Science 300(5620):805-8
8) Askree SH, et al.  (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63
9) Sinturel F, et al.  (2012) Activation of 5'-3' exoribonuclease Xrn1 by cofactor Dcs1 is essential for mitochondrial function in yeast. Proc Natl Acad Sci U S A 109(21):8264-9
10) Haimovich G, et al.  (2013) Gene expression is circular: factors for mRNA degradation also foster mRNA synthesis. Cell 153(5):1000-11