SUA5/YGL169W Summary Help

Standard Name SUA5 1
Systematic Name YGL169W
Feature Type ORF, Verified
Description Protein involved in threonylcarbamoyl adenosine biosynthesis; Sua5p and Qri7p are necessary and sufficient for RNA t6A modification in vitro; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family; binds single-stranded telomeric DNA and null mutant has abnormal telomere length (1, 2, 3, 4, 5)
Name Description Suppressor of Upstream AUG 1
Chromosomal Location
ChrVII:186059 to 187339 | ORF Map | GBrowse
Gbrowse
Genetic position: -104.06 cM
Gene Ontology Annotations All SUA5 GO evidence and references
  View Computational GO annotations for SUA5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
reduction of function
repressible
Large-scale survey
null
reduction of function
Resources
15 total interaction(s) for 14 unique genes/features.
Genetic Interactions
  • Phenotypic Enhancement: 9
  • Phenotypic Suppression: 2
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 426
Molecular Weight (Da) 46,538
Isoelectric Point (pI) 7.23
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:186059 to 187339 | ORF Map | GBrowse
SGD ORF map
Genetic position: -104.06 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1281 186059..187339 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003137
References cited on this page View Complete Literature Guide for SUA5
1) Hampsey M, et al.  (1991) Extragenic suppressors of a translation initiation defect in the cyc1 gene of Saccharomyces cerevisiae. Biochimie 73(12):1445-55
2) Na JG, et al.  (1992) Isolation and characterization of SUA5, a novel gene required for normal growth in Saccharomyces cerevisiae. Genetics 131(4):791-801
3) El Yacoubi B, et al.  (2009) The universal YrdC/Sua5 family is required for the formation of threonylcarbamoyladenosine in tRNA. Nucleic Acids Res 37(9):2894-909
4) Meng FL, et al.  (2009) Sua5p a single-stranded telomeric DNA-binding protein facilitates telomere replication. EMBO J 28(10):1466-78
5) Wan LC, et al.  (2013) Reconstitution and characterization of eukaryotic N6-threonylcarbamoylation of tRNA using a minimal enzyme system. Nucleic Acids Res 41(12):6332-46